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糖尿病脂肪肝患者网膜素-1与脂联素、炎症因子关系研究

邵金双   

  1. 天津医科大学三中心临床学院
  • 收稿日期:2013-06-03 修回日期:2013-09-01 出版日期:2013-12-15 发布日期:2013-12-15
  • 通讯作者: 邵金双

Association of Plasma Omentin-1Levels with Adiponectin and Inflammatory Cytokines in Diabetic Patients with Fatty Liver

SHAO Jin shuang   

  1. The Third Affiliated Hospital of Tianjin Medical University
  • Received:2013-06-03 Revised:2013-09-01 Published:2013-12-15 Online:2013-12-15
  • Contact: SHAO Jin shuang

摘要:

【摘要】  目的  研究2型糖尿病(T2DM)合并非酒精性脂肪性肝病(NAFLD)患者血浆网膜素-1与脂联素和肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、高敏C反应蛋白(hs-CRP)等炎症因子的关系。  方法  采用酶联免疫吸附法(ELISA)检测T2DM合并NAFLD组(A组,63例)、T2DM不合并NAFLD组(B组,63例)和正常对照组(C组,70例)的血浆网膜素-1和脂联素水平,同时测定上述3组生化指标及炎症指标(hs-CRP、TNF-α和IL-6),应用相关分析和多元回归分析方法分析血浆网膜素-1与脂联素及炎症指标的关系,应用Logistic回归分析T2DM合并NAFLD的影响因素。  结果  A组血浆网膜素-1(27.02±2.82)μg/L、脂联素水平(11.98±3.63)mg/L低于B组[分别为(31.52±2.81)μg/L(15.85±3.28)mg/L]和C组[分别为(35.92±2.80)μg/L、( 19.88±3.44)mg/L],且B组低于C组(P<0.01),A组血浆网膜素-1水平与脂联素、高密度脂蛋白胆固醇(HDL-C)正相关,与TNF-α、IL-6、空腹血(FBG)、胰岛素抵抗指数(HOMAIR)、内脏脂肪面积、腰围、腰臀比(WHR)、游离脂肪酸(FFA)负相关(P<0.05或P<0.01);多元逐步线性回归分析表明脂联素、TNF-α、IL-6是血浆网膜素-1水平的影响因素。Logistic回归分析显示网膜素-1、hs-CRP、内脏脂肪面积及游离脂肪酸(FFA)是T2DM合并NAFLD的独立影响因素(P<0.01)。  结论   T2DM患者合并NAFLD与网膜素-1降低相关,网膜素-1表达可能会受到脂联素及炎症因子的影响。

关键词: 糖尿病, 2型, 脂肪肝, 炎症, 脂肪因子, 网膜素-1, 脂联素

Abstract:

[Abstract]   Objective   To investigate the relationship of omentin-1with adiponectin and inflammatory cytokines in
type 2diabetes (T2DM) patients with nonalcoholic fatty liver disease (NAFLD).  Methods   The serum levels of omentin-1 and adiponectin were assayed by enzyme-linked immunosorbent assay (ELISA) in patients of T2DM with NAFLD (group A,n=63), T2DM without NAFLD (group B,n=63)and normal control group (group C, n=70). At the same time the biochemical markers and inflammatory marker, such as tumor necrosis factor (TNF)-α, high-sensitivity C-reactive protein (hs-CRP) and interleukin 6(IL-6) were detected in three groups. The correlation analysis and multiple regression analysis were used to detect the association of omentin-1with adiponectin and inflammatory markers. The logistic regression was used to analyze factors influencing NAFLD in patients with T2DM.   Results   The serum levels of omentin-1 and adiponectin were significantly lower in group A [ (27.02±2.82) μg/L and (11.98±3.63) mg/L] than those of group B [(31.52±2.81) μg/L and (15.85±3.28)mg/L] and group C [(35.92±2.80) μg/L and (19.88±3.44) mg/L], and there were significantly lower levels of them in group B than those of group C (P<0.01). The plasma omentin-1level was positively correlated with adiponectin and high density li-poprotein (HDL-C) in group A. Also the plasma omentin-1level was negatively correlated with TNF-α, IL-6, fasting blood glucose (FBG), homeostasis model assessment of insulin resistance (HOMA-IR), visceral adipose tissue, waist, waist -to-hip ratio (WHR) and free fatty acid in group A (P<0.05orP<0.01). Multiple stepwise regression analysis showed that adiponectin, TNF-αand IL-6were independent factors influencing the level of plasma omentin-1. Logistic regression analysis showed that omentin-1was one of independent factors influencing T2DM combined with NAFLD (P<0.01).   Conclusion   The incident of NAFLD in T2DM patients is related to the lower level of omentin-1, which may be influenced by adiponectin and inflammatory factors.

Key words: diabetes mellitus, type2, fatty liver, inflammation, adipokines, Omentin-1, adiponectin