天津医药

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荷丹片对动脉粥样硬化大鼠氧化应激的影响

周玉娟1,任明2,刘莉3,张喆4,李少春2   

  1. 1. 河北大学医学部基础医学院
    2. 河北大学
    3. 河北大学医学部基础医学院药理教研室
    4. 河北大学医学部
  • 收稿日期:2011-05-25 修回日期:2011-11-24 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 任明

The Effects of Hedan tablet on Oxidant Stress in Atherosclerosis Rats

  • Received:2011-05-25 Revised:2011-11-24 Published:2012-07-15 Online:2012-07-15
  • Contact: Ming Ren

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摘要： 目的 观察荷丹片对动脉粥样硬化大鼠氧化应激的影响。方法 48只雄性SD大鼠随机分为正常对照组、模型组、荷丹片高(3g.kg-1.d-1)、中(1.5g.kg-1.d-1)、低(0.5g.kg-1.d-1)剂量干预组及辛伐他汀组。正常组给予普通鼠饲料喂养，其余各组使用高脂饲料造模。12周后取各组大鼠腹主动脉电子显微镜下观察主动脉形态学变化；腹静脉取血测定各组大鼠SOD、MDA及NO含量。结果 荷丹片可以升高血清SOD、NO水平、降低血清MDA水平，与模型组相比差异有统计学意义(P＜0.05)，其抗AS作用呈剂量依赖性。高剂量荷丹片作用最为显著与辛伐他汀作用比较两者差异无统计学意义。荷丹片可以减轻主动脉动脉粥样硬化病理组织损伤。结论 高剂量荷丹片通过减轻AS大鼠氧化应激发挥其抗动脉粥样硬化的作用。

关键词: 荷丹片, 动脉粥样硬化, 氧化应激, 大鼠

Abstract: Objective To evaluate the effect of Hedan tablet on oxidant stress in atherosclerosis rats. Methods Forty-eight healthy male rats were randomly divided into the normal control group、the model group、the three Hedan tablet treated groups(3g.kg-1.d-1 、1.5g.kg-1.d-1、0.5g.kg-1.d-1)and the simvastatin treated group.Hyperlipidie feed was adopted to build the athersclerosis animal model.Rats were sacrificed 12 weeks later,their histopathological changes in abdominal aorta were observed by electronic microscope；serum SOD and NO were determined by by colorimetry, and serum MDA level was determined by Thiobarbituric acid method. Results In comparing with the model group, Hedan tablet reduced the serum level of MDA as well as increased the serum level of SOD、NO(P＜0.05)。Hedan tablet treatment(3g.kg-1.d-1) showed the best effect of inhibiting the atherosclerosis formation in the three Hedan tablet treated groups. Hedan tablet showed actions in lessening the atherosclerosis lesion. Conclusion Hedan tablet treatment(3g.kg-1.d-1) can inhibit the atherosclerosis formation in experimental rats .Its mechanism might be related with its anti-oxidation effect.

Key words: Hedan tablet, atherosclerosis, oxidant stress, rat