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MitoKATP通道开放对重症急性胰腺炎大鼠心肌保护的实验研究

田影   

  1. 天津中医药大学第二附属医院
  • 收稿日期:2011-11-14 修回日期:2012-06-19 出版日期:2012-11-15 发布日期:2012-11-15
  • 通讯作者: 田影

The Myocardial Protection of Opening MitoKATP Channel in Rats with Severe Acute Pancreatitis The Myocardial Protection of Opening MitoKATP Channel in Rats with Severe Acute Pancreatitis

Ying Tian   

  • Received:2011-11-14 Revised:2012-06-19 Published:2012-11-15 Online:2012-11-15
  • Contact: Ying Tian

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摘要： 目的：探讨线粒体ATP敏感性钾通道（MitoKATP通道）开放对重症急性胰腺炎（SAP）心肌的保护作用及机制。方法：清洁级雄性SD大鼠36只随机分为4组：K组假手术；M组建立SAP大鼠模型；D组和H组分别给予连续腹腔注射二氮嗪(DZ)、DZ+5-羟基葵酸盐（5-HD）3d后建立SAP大鼠模型。观察4组大鼠术后24 h血清肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（LDH）水平；分光光度法测定心肌组织Na+-K+-ATPase活性；流式细胞仪检测心肌细胞线粒体线粒体膜电位(?ψm)变化；原位末端标记（TUNEL）法检测心肌细胞凋亡情况；留取部分心脏、胰腺组织行病理学检查。结果：M组CK-MB、LDH水平、心脏病理评分及凋亡指数较D组升高，Na+-K+ -ATPase活性及?ψm较D组降低，差异均有统计学意义（P < 0.01），但M组上述指标与H组比较差异均无统计学意义（P > 0.05）。结论：MitoKATP通道开放对SAP引起的心肌损伤有保护作用。

关键词: 线粒体敏感性钾通道, 重症急性胰腺炎, 大鼠, 心肌保护

Abstract: Objective: To investigate the role of MitoKATP in rats with SAP and its mechanism of myocardial protection. Methods: 36 healthy SD rats were randomly divided into 4 groups: K group was given sham operation; M group was established SAP model in rats; Respectively consecutive intraperitoneal injection of DZ and DZ+ 5-HD for 3 days,D and H groups were made to SAP model too. Detected the levels of serum CK-MB and LDH at 24 hours after the operation.Applied Spectrophotometry to detect Na+-K+-ATPase activity of myocardial tissue.The alteration of mitochondrial membrane potential was detected by flow cytometry.The technique of TUNEL was used to detect myocardial apoptosis. Heart and pancreas was stored to perform histopathologic examination. Results: Compared with the D group,the levels of serum CK-MB and LDH,the heart pathological scores and the myocardial apoptosis index were higher in M group (P<0.01),while the Na+-K+-ATPase activity and mitochondrial membrane potential decreased in M group(P<0.01)。But there were no significant difference with Mand H group（P>0.05）. Conclusion: Opening MitoKATP Channel had myocardial protective effect in Rats with SAP.

Key words: MitoKATP, Severe acute pancreatitis, rats, myocardial protection