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电针对吗啡耐受大鼠辣椒素受体磷酸化水平变化的影响

黄美娜1,郑宇欣1,于永浩1,王国林2   

  1. 1. 天津医科大学总医院
    2. 天津医大总医院
  • 收稿日期:2011-03-06 修回日期:2011-03-16 出版日期:2011-06-15 发布日期:2011-06-15
  • 通讯作者: 黄美娜

The effect of electroacupuncture on changes of the phosphorylation of capsaicin receptor in chronic morphine tolerance rats

  • Received:2011-03-06 Revised:2011-03-16 Published:2011-06-15 Online:2011-06-15

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摘要： 目的：探讨电针对大鼠吗啡耐受和辣椒素受体成员-瞬时受体电位辣椒素亚家族成员1（transient receptor potential vanilloid subfamily member 1,TRPV1）磷酸化水平变化的影响。方法：24只健康雄性SD大鼠随机分为3组（n=8）：分别经鞘内给予生理盐水10μL为N组，吗啡10μg为M组，吗啡10μg并于每天首次给药后电针刺激大鼠足三里和阳陵泉两穴2 Hz为H组，3组均一日2次给药，连续7d。用50%机械缩爪阈值及热板缩爪潜伏期评价大鼠机械痛敏及热痛敏。于给药后第8天取L4～5背根神经节（DRG）,采用Western blot分析其中TRPV1总蛋白及TRPV1磷酸化水平。 结果：M组在连续给予吗啡后形成稳定的吗啡耐受。2 Hz电针处理可缓解吗啡耐受形成。M组TRPV1总蛋白表达高于N组、H组，差异有统计学意义，N组与H组比较差异无统计学意义。M组背根神经节内TRPV1磷酸化水平增加，H组TRPV1磷酸化水平与M组相比明显减少，差异均有统计学意义。 结论：低频2 Hz电针可抑制慢性吗啡耐受及耐受相关痛觉过敏，可能与抑制背根神经节内TRPV1磷酸化有关。

关键词: 电针, 吗啡耐受, 辣椒素受体, 磷酸化, 背根神经节

Abstract: Objective：To investigate the effect of electroacupuncture(EA) on morphine tolerance of rats and on changes of the phosphorylation of transient receptor potential vanilloid subfamily member 1（TRPV1）,which is a member of capsaicin receptor. Method:The 24 healthy male SD rats were randomized into 3 groups (n=8): they were administered respectively intrathecally 10 L saline for group N; 10 g morphine for group M; 10g morphine and 2HzEA on "Zu-San-Li" and "Yang-Ling-Quan" after the first administration of morphine every day for group H.Administration of the three groups were all twice daily for 7days.Mechanical withdrawal threshold (MWT) and thermal paw withdrawal latency (PWL) of rats was examined to evaluate their mechanical hyperalgesia and heat hyperalgesia. Take the L4~5 dorsal root ganglion(DRG) on the 8 day of administration ,and the expression of total TRPV1 and phosphorated TRPV1 in DRG was measured by western-blot.Result: The rats in group M were developed morphine tolerance .2Hz EA can alleviated the formation of morphine tolerance.The expression of total TRPV1 in group M were significantly higher than that in group N and group H,while there's no significant difference between group N and group H.The phosphorylation of TRPV1 in DRG of group M were increased significantly,while the expression of phosphorylated TRPV1 in group H were significantly lower compared with that of group M.Conclusion:2Hz EA has inhibitory effect on morphine tolerance and tolerance-associated thermal hyperalgesia,it may be through the inhibition of phosphorylation of TRPV1 in DRG.

Key words: Electroacupuncture(EA), Morphine tolerance, TRPV1, Phosphorylation, dorsal root ganglion(DRG)