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重度子痫前期患者外周血单个核细胞颗粒酶B mRNA表达及意义

魏军1,李雪玲2,孙羽1,郑泽华1   

  1. 1. 中国医科大学附属盛京医院
    2. 安徽省安庆市立医院
  • 收稿日期:2011-06-13 修回日期:2011-09-09 出版日期:2012-03-15 发布日期:2012-03-15
  • 通讯作者: 魏军

The Expression of the Granzym B mRNA in Peripheral Blood Mononuclear Cells and its Clinical Signisicance in Severe Preeclamptic Patients

  • Received:2011-06-13 Revised:2011-09-09 Published:2012-03-15 Online:2012-03-15
  • Contact: Jun WEI

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摘要： 目的：探讨颗粒酶B在子痫前期发病中作用。方法：2007年12月-2009年6月中国医科大学附属盛京医院产科重度子痫前期患者32例，正常孕妇32例。采用逆转录聚合酶链反应方法检测子痫前期患者外周血单个核细胞颗粒酶B mRNA的表达，并与平均动脉压进行相关性分析。结果：(1)子痫前期组颗粒酶B mRNA相对表达强度为1.297±0.239，对照组相对表达强度为0.792±0.210。重度子痫前期组GzmB mRNA表达明显高于对照组，t=8.963 P<0.001.(2) 重度子痫前期组孕妇平均动脉压为(133±5) mm Hg(1mmHg=0.133 kPa), 重度子痫前期孕妇外周血中单个核细胞PFN mRNA表达水平与平均动脉压呈显著正相关（r =0693，P<0.001）。结论：子痫前期患者外周血单个核细胞中颗粒酶BmRNA表达水平增高，并与平均动脉压呈显著正相关，可能参与了重度子痫前期的发病过程。 关键词：颗粒酶; 子痫前期，血管内皮细胞

关键词: 子痫前期, 颗粒酶B, 内皮细胞

Abstract: Objectives】: To dectect the Expression of the Granzym(Gzm) B mRNA in Peripheral blood mononuclear cells of preeclamptic patients. Methods: 32 cases of preeclampsia and 32 cases of normal pregnancy were selected as in random. RT-PCR is adopted to detect the expression of Gzm B mRNA in the peripheral blood mononuclear cells and its correlation with mean arterial pressure was analyzed in severe preeclamptic patients. Results:(1) The expression of Granzym mRNA level in severe preeclampsia group was 1.297±0.239， significant higher than those of control group 0.792±0.210， t=8.963 ,P<0.001. (2)Correlation analysis: The mean blood pressure in severe preeclampsia group was (133±5) mm Hg(1mmHg=0.133 kPa).There was significant positive correlation between level of Gzm B mRNA in PBMC and mean blood pressure in severe preeclamptic patients(r=0.693，P<0.001.).Conclusion: Expression of Granzym B was significantly increased in severe preeclampsia , and was of significant positive correlation with the mean blood pressure. Granzym B may participate in the pathogenesis of severe preeclampsia.

Key words: Preeclampsia, Granzym B, endothelium