天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (4): 337-339.doi: 10.11958/j.issn.0253-9896.2015.04.001

• 细胞与分子生物学 •    下一篇

叶酸对体外氧化损伤血管内皮细胞作用的剂量效应研究

高玉霞1，崔杉杉2，李文2，王鹏燕2，肖艳宇2，黄国伟2△   

  1. 1天津医科大学总医院心内科（邮编300052）；2天津医科大学公共卫生学院营养与食品卫生学系
  • 收稿日期:2014-09-30 修回日期:2014-11-16 出版日期:2015-04-15 发布日期:2015-04-13
  • 通讯作者: 黄国伟 E-mail:tjgwhuang@126.com
  • 作者简介:高玉霞（1963），女，副教授，博士，主要从事营养与动脉粥样硬化研究
  • 基金资助:
    国家自然科学基金资助项目（81373002）

The dose-effect relationship of folic acid on human umbilical vein endothelial cells in vitro

GAO Yuxia1，CUI Shanshan2，LI Wen2，WANG Pengyan2，XIAO Yanyu2，HUANG Guowei2Δ   

  1. 1 Department of Cardiology, General Hospital of Tianjin Medical University, Tianjin 300052, China; 2 Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University
  • Received:2014-09-30 Revised:2014-11-16 Published:2015-04-15 Online:2015-04-13
  • Contact: HUANG Guowei E-mail:tjgwhuang@126.com

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摘要： 摘要：目的探讨叶酸对氧化低密度脂蛋白（ox-LDL）损伤的人脐静脉内皮细胞（HUVEC）的保护作用。方法正常HUVEC 按叶酸剂量不同分为叶酸缺乏组（FA-def）、FA-15、FA-50、FA-100、FA-200、FA-500 nmol/L 组。正常 HUVEC 培养液中加入ox-LDL 使其终浓度为120 mg/L，造成氧化损伤，并与7 个不同叶酸浓度（0、15、60、150、225、 300、375 nmol/L）共同作用24 h 后，维持叶酸浓度不变更换无ox-LDL 培养液继续作用72 h，并于24、48、72、96 h 后收集氧化损伤细胞。采用倒置显微镜观察其形态改变，MTT 法检测细胞活力改变。结果不同浓度叶酸（0、15、50、 100、200、500 nmol/L）对正常HUVEC 无明显促进或抑制作用（P＞0.05）；叶酸干预氧化损伤的HUVEC 作用24 和48 h 后，与ox-FA-def 组相比，ox-FA-150、ox-FA-225、ox-FA-300、ox-FA-375 nmol/L 组均可以促进细胞增殖（P＜ 0.05）；作用72 和96 h，与ox-FA-def 组相比，叶酸ox-FA-60、ox-FA-150、ox-FA-225、ox-FA-300、ox-FA-375 nmol/L 组均可以促进细胞增殖（P＜0.05）。结论150、225、300 和375 nmol/L 叶酸干预较短时间（24 或48 h）或60 nmol/L 叶酸干预较长时间（72 或96 h）均能降低ox-LDL 对HUVEC 的氧化损伤，且其作用有明显剂量效应关系。

关键词: 叶酸, 剂量效应关系, 药物, 内皮细胞, 脂蛋白类, LDL

Abstract: Abstract：Objective To investigate the protective effects of folic acid on the oxidative damage that ox-LDL (oxi⁃ dized low-density lipoprotein receptor 1) render to human umbilical vein endothelial cells (HUVEC). Methods HUVECs were injured by ox-LDL (120 mg/L) for 24 h while they were incubated with various concentration of folic acid (0，15, 60, 150, 225, 300, 375 nmol/L). Then HUVECs were cultured in media contains same concentration of folic acid but without ox- LDL for 72 hours. Finally, HUVECs were harvested after 24, 48, 72 and 96 h. The morphological changes were observed us⁃ ing inverted microscope and cell viability were examined by MTT. Results Various concentrations of folic acid (0,15, 50, 100, 200 and 500 nmol/L) has no obvious promotion or inhibition effect in growth of normal HUVEC (P＞0.05). However, compared with the ox-FA-def group, 150, 225, 300 and 375 nmol/L of folic acid promoted proliferation of HUVECs with 96 and 120 hours of incubations (P < 0.05). Folic acid of 60, 150, 225, 300 and 375 nmol/L promoted the proliferation of HUVECs with 72 h and 96 hours of incubation (P < 0.05). Conclusion High dose folic acid can reduce the ox-LDL oxida⁃ tive damage on HUVEC in a concentration dependent manner.

Key words: folic acid, dose-response relationship, drug, endothelial cells, lipoproteins, LDL