天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (3): 263-266.doi: 10.11958/j.issn.0253-9896.2015.03.011

• 实验研究 • 上一篇    下一篇

急性切口痛大鼠血浆及上消化道胃动素表达的变化

赵军 1， 徐磊 2△， 张瑜 3， 冯全胜 2   

  1. 1天津医科大学三中心临床学院 （邮编 300170）； 2天津市第三中心医院重症医学科、 天津市人工细胞重点实验室； 3天津医科大学附属肿瘤医院麻醉科
  • 收稿日期:2014-09-26 修回日期:2014-11-07 出版日期:2015-03-15 发布日期:2015-03-15
  • 通讯作者: 徐磊 E-mail:nokia007008@163.com
  • 作者简介:赵军 （1979）， 男， 硕士在读， 主要从事急性疼痛及胃肠动力基础研究
  • 基金资助:
    天津市卫生局科技基金资助项目 （2012KY03）

Changes of motilin concentrations in plasma and upper gastrointestinal tract in rat model of acute incisional pain

ZHAO Jun1， XU Lei2△， ZHANG Yu3， FENG Quansheng2   

  1. 1 The Third Central Clinical College of Tianjin Medical University， Tianjin 300170， China； 2 Department of Critical Care Medicine, the Third Central Hospital of Tianjin； 3 Department of Anesthesiology， Tianjin Medical University Cancer Institute and Hospital

  • Received:2014-09-26 Revised:2014-11-07 Published:2015-03-15 Online:2015-03-15
  • Contact: XU Lei E-mail:nokia007008@163.com

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摘要： 目的 观察急性切口痛大鼠血浆、 胃体及十二指肠胃动素 （MTL） 水平的变化。方法 SPF 级健康雄性 SD大鼠 156 只， 6～8 周龄， 体质量 180～220 g， 采用随机数字表法将其分为 2 组（n=78）： 对照组（C 组）和切口痛组（P组）。P 组行右足跖肌切口。2 组先随机各取 6 只大鼠， 于术前 24 h（T0）、 术后 1 h（T1）、 6 h（T2）、 24 h（T3）、 48 h（T4）、72 h （T5） 测定机械缩足阈值 （PWMT） 与热缩足反射潜伏期 （PWTL）。并于上述每个时点， 2 组分别取 12 只大鼠， 断头处死， 提取血浆、 胃体及十二指肠组织， 采用 ELISA 法测定 MTL 水平。结果 与 C 组比较， P 组 T1~T4时 PWMT 降低， PWTL 缩短， 血浆、 胃体 MTL 水平降低， 而十二指肠 MTL 水平升高 （P＜0.05）， T0、 T5时差异无统计学意义； P 组血浆 MTL 水平与 PWMT、 PWTL 呈正相关 （r 分别为 0.952、 0.879， P＜0.01）， P 组胃体 MTL 水平与 PWMT、 PWTL 呈正相关性 （r 分别为 0.970、 0.931， P＜0.01）； 十二指肠 MTL 水平与 PWMT、 PWTL 呈负相关性 （r 分别为-0.991、 -0.975， P＜0.01）。结论 急性切口痛可使大鼠血浆、 胃体 MTL 水平降低， 而使十二指肠 MTL 水平升高。

关键词: 促胃动素, 胃, 十二指肠, 血浆, 急性切口痛

Abstract: Objective To observe changes of motilin(MTL) levels in gastric body, duodenum and plasma in rat model of acute incisional pain．Methods A total of 156 healthy male adult SD rats， weighing 180-220 g， were randomized into two groups: control group (group C， n=78) and incisional pain group (group P， n=78)， Rats in P group received incision on the right plantaris. Values of paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) at dif⁃ ferent time points of 24 hours before operation (T0) and 1 hour (T1)， 6 hours (T2)， 24 hours(T3)， 48 hours (T4) and 72 hours (T5) after operation were measured in six rats chosen randomly from each group. Twelve rats were chosen from each group at T0-5, and sacrificed. The MTL levels in plasma, the mucosal tissues of gastric body and duodenum were detected by ELISA. Results Compared with group C, PWMT and PWTL were significantly decreased at T1-4 in group P. The MTL levels were sig⁃ nificantly decreased in plasma and gastric body (P＜0.05)．The MTL level was significantly increased at T1-4 in duodenum (P＜0.05)， and no significant changes were found at T0 and T5 in P group(P > 0.05). The plasma MTL levels were positively correlated with PWMT and PWTL (r=0.952， r=0.879， respectively， P＜0.01) in P group. The MTL levels in gastric body were positively correlated with PWMT and PWTL（r=0.970， r=0.931， respectively， P＜0.01) in P group. The MTL levels were neg⁃ atively correlated with PWMT and PWTL(r=- 0.991， r=- 0.975， respectively， P＜0.01) in duodenum in P group. Conclusion The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo⁃ denum．model of acute incisional pain

The MTL levels in plasma and gastric body are decreased in rat model of acute incisional pain, and increased in duo⁃ denum．

Key words: motilin, stomach, duodenum, plasma, acute incisional pain