天津医药

天津医药 ›› 2015, Vol. 43 ›› Issue (8): 860-863.doi: 10.11958/j.issn.0253-9896.2015.08.008

• 实验研究 • 上一篇    下一篇

Roux-en-Y 胃旁路术对2 型糖尿病大鼠脂联素及胰岛凋亡蛋白表达的影响

柴芳，高向楠，王俊，李强，赵树鹏   

  1. 辽宁锦州市，辽宁医学院附属第一医院普外科
  • 收稿日期:2015-03-09 修回日期:2015-04-01 出版日期:2015-08-15 发布日期:2015-08-15
  • 通讯作者: 赵树鹏 E-mail:chaifang730919@sina.com
  • 作者简介:柴芳（1973），男，副主任医师，博士研究生，主要从事胃旁路手术治疗2型糖尿病的基础研究
  • 基金资助:
    辽宁省自然科学基金资助项目（2013022044）；辽宁医学院博士科研启动基金项目（Y2012B017）

The effects of Roux-en-Y gastric bypass on expressions of adiponectin and pancreatic islets related apoptotic proteins in type 2 diabetic rats

CHAI Fang, GAO Xiangnan, WANG Jun, LI Qiang, ZHAO Shupeng   

  1. The First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, China
  • Received:2015-03-09 Revised:2015-04-01 Published:2015-08-15 Online:2015-08-15
  • Contact: ZHAO Shupeng E-mail:chaifang730919@sina.com

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摘要： 目的通过研究脂联素及胰岛凋亡相关蛋白的变化，探讨Roux-en-Y 胃旁路术（RYGB）减少胰岛细胞凋亡的机制。方法60 只SD 大鼠随机分为RYGB 组、2 型糖尿病（T2DM）组和正常对照（NC）组，每组20 只。NC 组喂食正常饲料；为了建立T2DM 动物模型，T2DM 组和RYGB 组大鼠喂食高脂饮食（22.19 kJ/g）3 周，并于第13 天经腹腔注入链脲佐菌素（STZ，30 mg/kg）。造模成功后，RYGB 组大鼠行RYGB 手术、T2DM 及NC 组行假手术。术前、术后7、14 及21 d 测定体质量；术前及术后21 d 测定空腹血糖，ELISA 法测定血浆脂联素水平；术后21 d 免疫组化 SABC 法检测胰岛Bcl-2、Caspase 8、Caspase 9 表达。结果术前3 组体质量差异无统计学意义；RYGB 和T2DM 组血糖明显高于NC 组，脂联素水平明显低于NC 组（P < 0.05）。与T2DM 及NC 组相比，术后RYGB 组体质量明显降低。与T2DM 组相比RYGB 组术后21 d 时血糖明显降低、脂联素水平明显升高（P < 0.05），RYGB 组与NC 组相比差异无统计学意义。与T2DM 组相比RYGB 组术后胰岛Bcl-2 表达明显升高，Caspase 8、Caspase 9 表达明显降低（P < 0.05）。结论RYGB 术后可以使脂联素水平升高、Bcl-2 表达增加，Caspase 8、Caspase9 表达减少；RYGB 术可能通过线粒体通路减少胰岛细胞凋亡。

关键词: 糖尿病, 实验性, 胃旁路术, 吻合术, Roux-en-Y, 脂联素, 胰岛, 细胞凋亡

Abstract: Objective To explore the anti-apoptotic mechanism of Roux-en-Y gastric bypass (RYGB) through exam⁃ ine the postoperative change of adiponectin levels and expressions of pancreatic islets relative apoptotic protein. Methods Sixty SD rats were randomly allocated to RYGB group (n=20), type 2 diabetes mellitus group (T2DM, n=20) and normal con⁃ trol group (NC, n=20). Rats in the NC group were fed with normal diet. In order to make type 2 diabetic rat models, the rats in the T2DM and RYGB groups were fed with high fat diet (22.19 kJ/g) combined with administration of intraperitoneal strep⁃ tozotocin injection (STZ, 30 mg/kg) on the 13th day of high fat diet. RYGB operation were performed in RYGB group and sham-operation were performed in the T2DM and NC groups when diabetic model was contructed. Rats were weight preoper⁃ atively and at the 7th, 14th, 21st days after operations. Fasting plasma glucose and adiponectin (ELISA) were measured preoper⁃ atively and at 21st day postoperatively. Protein expressions of Bcl-2，Caspase 8 and Caspase 9 in pancreatic islets were ex⁃ amined by immunohistochemistry at the 21st day postoperatively. Results Body weights do not vary significantly among three groups preoperatively. Compared to rats in the NC group, fast plasma glucose level was higher but adiponectin was low⁃ er in rats in RYGB and T2DM groups. Body weights of rats in RYGB group decreased significantly compared to those of rats in NC and T2DM groups postoperatively. Compared to rats in T2DM group, fasting glucose level was lower while adiponectin concentrations was higher in rats in RYGB group but no differences of these parameters were seen in rats in NC group at the 21st day postoperatively. Expression of Bcl-2 in RYGB group was significantly elevated while expressions of Caspase 8 and Caspase 9 were significantly decreased compared to those in T2DM group postoperatively. Conclusion Adiponectin levels was elevated; expressions of Bcl-2 was increased; expressions of Caspase 8, Caspase 9 were decreased upon RYGB opera⁃ tion in T2DM model. RYGB might reduce pancreatic islets apoptosis through mitochondrial pathway.

Key words: diabetes mellitus, experimental, gastric bypass, anastomosis, Roux-en-Y, adiponectin, islets of langerhans, apoptosis