天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (1): 46-49.doi: 10.11958/51942

• 细胞与分子生物学 • 上一篇    下一篇

重组人白细胞介素-11对肺腺癌A549 细胞增殖、 迁移和侵袭的影响

肖荣， 康马飞△， 骆梅青， 董翠梅， 刘秀丽   

  1. 桂林医学院附属医院肿瘤内科 （邮编541001）
  • 收稿日期:2015-06-25 修回日期:2015-09-17 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: △通讯作者 E-mail:kmfgl@163.com E-mail:yiyeguzhouzhixiao@163.com
  • 作者简介:肖荣 （1990）， 女， 硕士研究生， 主要从事肿瘤的基础与临研究
  • 基金资助:
    桂林市科技攻关项目 （20150126-1-2）

Effects of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of pulmonary adenocarcinoma A549 cells

XIAO Rong, KANG Mafei △, LUO Meiqing, DONG Cuimei, LIU Xiuli   

  1. Department of Medical Oncology, the Affiliated Hospital of Guilin Medical College, Guilin 541001, China
  • Received:2015-06-25 Revised:2015-09-17 Published:2016-01-15 Online:2016-01-15
  • Contact: △Corresponding Author E-mail: kmfgl@163.com E-mail:yiyeguzhouzhixiao@163.com

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摘要： 摘要:目的 观察重组人白细胞介素-11 （rhIL-11） 对肺腺癌 A549 细胞增殖、 迁移和侵袭的影响， 并初步探讨其影响机制。方法 分别以 0、 10、 20、 50、 100 μg/L 终浓度的 rhIL-11 作用于 A549 细胞， MTT 法测定 A549 细胞增殖程度； 用 0、 10、 40、 80 μg/L 终浓度的 rhIL-11 作用于 A549 细胞， 划痕实验和 Transwell 侵袭实验测定 A549 细胞的迁移和侵袭能力； Western blot 检测基质金属蛋白酶 （MMP） -2 和 MMP-9 蛋白水平的表达。结果 rhIL-11 对 A549 细胞增殖能力无影响； rhIL-11 作用于 A549 细胞后， 细胞的迁移和侵袭能力显著增强； rhIL-11 能显著上调 MMP-2 和 MMP-9 的表达， 且表达随 rhIL-11 浓度的增高而增高 （P < 0.05）。结论 rhIL-11 能够促进 A549 细胞的迁移和侵袭， 促进 MMP-2、 MMP-9 上调为其可能的机制之一。

关键词: 白细胞介素 11, 癌, 非小细胞肺, 细胞迁移分析, 肿瘤侵润, 基质金属蛋白酶 2, 基质金属蛋白酶 9, 重组人白细胞介素 11

Abstract: Objective To observe the effects of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of A549 cells, and the mechanism thereof. Methods Final concentrations of 0, 10, 20, 50 and 100 μg/L rhIL- 11 were added into pulmonary adenocarcinoma A549 cells. The cell proliferation was detected by MTT. The wound-healing, transwell migration assay were used to validate the capability of the migration and invasion of A549 cells. Matrix metallopro⁃ teinases (MMP)-2 and MMP-9 protein expressions were revealed by Western blot assay. Results The proliferation of A549 cells was not significantly changed by rhIL-11. The cell capability to migrate and invade was significantly increased 24 h af⁃ ter treatment with rhIL-11 (P < 0.05). The expression levels of MMP-2 and MMP-9 were significantly un-regulated, and which were increased with the increased concentrations of rhIL-11 (P < 0.05). Conclusion rhIL-11 can promote the migra⁃ tion and invasion of A549 cells, and the up-regulation of MMP-2 and MMP-9 expression might be one of the mechanisms.

Key words: interleukin-11, carcinoma, non-small-cell lung;, cell migration assays, neoplasm invasiveness, matrix me? talloproteinase 2, matrix metalloproteinase 9, rhIL-11