天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 178-181.doi: 10.11958/20150129

• 实验研究 • 上一篇    下一篇

不同灌注方式对大鼠小体积肝移植生存率的影响

杨超 1， 张雅敏 2△， 崔子林 2， 刘子荣 1， 史源 2， 沈中阳 2   

  1. 1天津医科大学一中心临床医学院 （邮编300192）； 2天津市第一中心医院
  • 收稿日期:2015-08-25 修回日期:2015-10-18 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: △Corresponding Author E-mail: zhangyamin@medmail.com.cn E-mail:zhangyamin@medmail.com.cn
  • 基金资助:
    国家自然科学基金面上项目(81370576)； 天津市应用基础与前沿技术研究计划(14JCYBJC24800)

Impact of different ways of perfusion on the survival rate of small-for-size liver transplantation in rats

YANG Chao1, ZHANG Yamin2△, CUI Zilin2, LIU Zirong1, SHI Yuan2, SHEN Zhongyang2   

  1. 1 First Center Clinical Institute, Tianjin Medical University, Tianjin 300192, China; 2 Tianjin First Center Hospital
  • Received:2015-08-25 Revised:2015-10-18 Published:2016-02-15 Online:2016-02-15
  • Contact: △通迅作者 E-mail： zhangyamin@medmail.com.cn E-mail:zhangyamin@medmail.com.cn

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摘要： 目的 探讨不同的灌注方式对大鼠小体积肝移植生存率的影响。方法 将 156 只 SD 大鼠随机分为经门静脉灌注组（GroupⅠ）和经腹主动脉灌注组（GroupⅡ）， 每组 78 只， 供、 受体各 39 只。GroupⅠ与 GroupⅡ在获取供肝后， 切除肝脏的左叶和中叶， 剩余约 30%体积进行移植， 术中记录供体和受体的体质量、 移植肝质量、 温缺血时间、供体手术时间、 冷缺血时间、 无肝期、 肝下下腔静脉阻断时间及受体手术时间相关指标， 比较 2 组大鼠术后 6 h、 1 d、 3 d 和 7 d 的血清丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平、 病理 HE 染色及 7 d 生存率。结果 2 组术中血清 ALT、 AST 均逐渐下降， 但是 GroupⅠ下降缓慢， 术后 24 h 高于 GroupⅡ（P＜0.05）。HE 染色提示 GroupⅠ术后早期肝组织显微结构损伤大， GroupⅠ的 7 d 中位生存期低于 GroupⅡ （Log-rank χ2=4.050， P=0.044）。结论 使用经腹主动脉灌注的方法建立大鼠小体积肝移植模型具有肝脏损伤小、 生存率高的优势。

关键词: 肝移植, 大鼠, Sprague-Dawley, 小体积肝移植, 灌注方式

Abstract: Objective To investigate the effects of different hepatic perfusion procedures for small-for-size liver transplantation in rats. Methods A total of 156 rats were randomly divided into two groups: portal vein perfusion group (groupⅠ, n=78) and abdominal aorta perfusion group (group Ⅱ, n=78). After harvesting graft, the left lobe of the liver and the middle lobe were resected and the remaining approximately 30% volumes of the liver were transplanted in group Ⅰ and group Ⅱ. The body weights of donor and acceptor, the weight of graft, the time of operating in donor, the cold ischemia time, anhepatic phase, the blocking time of inferior hepatic vena cava and the time of operating in receptor were recorded in two groups. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), pathological HE staining and 7-day survival rate in 6 h, 1 d, 3 d and 7 d after operation were compared between two groups. Results The serum levels of ALT and AST were decreased gradually in two groups, but the levels decreased slowly in group Ⅰ. The serum levels of ALT and AST were significantly higher in group Ⅰ than those of group Ⅱ (P＜0.05). HE staining showed greater damage of mi⁃ crostructure of liver tissue at early stage in group Ⅰ than that in group Ⅱ. The 7-day survival rate was lower in group Ⅰ than that of group Ⅱ (χ2=4.050， P=0.044). Conclusion There is a higher survival rate and mild liver damage in small-forsize liver transplantation in rats using perfusion by abdominal aorta.

Key words: liver transplantation, rats, Sprague-Dawley, small for size liver transplantation, perfusion