天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (2): 196-199.doi: 10.11958/59135

• 实验研究 • 上一篇    下一篇

心功能不全合并糖尿病大鼠模型的建立与评价

李田乐 1， 李彤 2△， 胡晓旻 2， 杨帆 2， 汪丽红 3， 杨玲 2   

  1. 1天津医科大学三中心临床学院 （邮编300170）； 2天津市第三中心医院； 3天津市港口医院
  • 收稿日期:2015-06-23 修回日期:2015-09-20 出版日期:2016-02-15 发布日期:2016-02-15
  • 通讯作者: △通讯作者 E-mail:litong_3zx@sina.com E-mail:1294170903@qq.com
  • 基金资助:
    天津市卫生局科技基金攻关资助项目 （12KG106）

Establishment and evaluation of rat model of cardiac insufficiency complicated with diabetes mellitus

LI Tianle1 , LI Tong 2△, HU Xiaomin2, YANG Fan2 , WANG Lihong3 , YANG Ling2   

  1. 1 The Third Central Clinical Medical College of Tianjin Medical University, Tianjin 300170, China; 2 Tianjin Third Central Hospital; 3 Tianjin Port Hospital
  • Received:2015-06-23 Revised:2015-09-20 Published:2016-02-15 Online:2016-02-15
  • Contact: △Corresponding Author E-mail: litong_3zx@sina.com E-mail:1294170903@qq.com

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摘要： 目的 制备一种高稳定性心功能不全合并糖尿病大鼠模型， 以期方便于临床和实验室研究。方法 取清洁级雄级 Wistar 大鼠 45 只， 随机分为 3 组， A 组 （正常组） 10 只， B 组 （腹主动脉缩窄组） 10 只， C 组 （腹主动脉缩窄合并糖尿病组） 25 只。首先对 B、 C 组大鼠采用腹主动脉缩窄的方法构建心功能不全模型， 缩窄 2 个月后， 将 C 组大鼠随机分为 5 个亚组， C1~5， 分别采用单次腹腔注射 40、 45、 50、 55、 60 mg/kg 链脲佐菌素 （STZ） 的方法诱导心功能不全合并糖尿病大鼠模型。实验过程中动态观察大鼠一般状况及体质量改变， 监测 STZ 注射后 72 h、 4 周大鼠血糖水平，并于 STZ 注射后 1 个月对各组大鼠进行心脏超声及心肌病理改变评价。结果 对比不同剂量， STZ 为 45 mg/kg 时，构建模型稳定性高且成模大鼠一般状况、 血糖水平、 心脏超声、 心肌组织镜下形态学较正常组发生明显变化， 符合心功能不全合并糖尿病改变。结论 采用腹主动脉缩窄 2 个月后腹腔单次注射 45 mg/kg STZ 的方法诱导心功能不全合并糖尿病动物模型， 方法简便可靠， 稳定性高。

关键词: 心功能不全, 糖尿病, 实验性, 腹主动脉缩窄, 疾病模型, 动物, 大鼠, Wistar

Abstract: Objective To establish a rat model of cardiac insufficiency complicated with diabetes mellitus (DM), and to meet the needs of clinical and laboratory studies. Methods Forty-five male specific pathogen free (SPF) rats were randomly divided into three groups: normal control group (A, n=10 ), coarctation of the aorta (AAC) group (B, n=10), AAC+DM group (C, n=25). The model of cardiac insufficiency with abdominal aortic constriction was establish in B and C groups. After two months of AAC, rats of group C were randomly divided into five subgroups and treated with different doses of streptozotocin (STZ) respectively(40, 45, 50, 55 and 60 mg/kg). The dynamic changes of general condition and weights were observed during the process of experiment. The blood glucose levels of 72 h and 4 week after STZ injection were detected. The echocardiograph and cardiac pathology changes were evaluated after 1 month of STZ injection. Results The general data including blood glucose levels, echocardiographic findings and myocardial tissue microscopic morphology were compared be⁃tween different doses of STZ groups. The 45 mg/kg STZ was considered for more stable model of cardiac dysfunction complicated with diabetes mellitus. Conclusion The rat model of cardiac insufficiency complicated with diabetes mellitus is established by single dose injection of 45 mg/kg STZ after two-month AAC, which is a simple, reliable and high stability method.

Key words: cardiac insufficiency, diabetes mellitus,experimental, abdominal aortic constriction, disease models, animal, rats, Wistar