天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (3): 311-313.doi: 10.11958/20150159

• 细胞与分子生物学 • 上一篇    下一篇

布南色林对H2O2引起PC12细胞损伤的神经保护作用研究

黄海潮,张小红,聂阳,刘经亮 巫玮   

  1. 1 广东食品药品职业学院（邮编 510520）；2中山大学肿瘤防治中心药学部
  • 收稿日期:2015-09-10 修回日期:2015-11-16 出版日期:2016-03-15 发布日期:2016-03-15
  • 通讯作者: 巫玮 E-mail:huanghac@gdyzy.edu.cn
  • 作者简介: 黄海潮（1982）， 男， 实验师， 硕士研究生， 主要从事神经药理研究
  • 基金资助:
    广东省医学科学技术研究基金

Neuroprotective effects of Blonanserin against H2O2-induced injuried in PC12 cells

HUANG Haichao, WU Wei, ZHANG Xiaohong, NIE Yang, LIU Jingliang, ZHOU Jie   

  1. 1 Guangdong Food and Drug Vocational College, Guangzhou 510520, China; 2 Department of pharmacy, SUN YAT-SEN University Cancer Center
  • Received:2015-09-10 Revised:2015-11-16 Published:2016-03-15 Online:2016-03-15
  • Contact: WU Wei E-mail:huanghac@gdyzy.edu.cn

PDF (PC)

3713

摘要： 目的 探讨布南色林对H2O2引起的PC12细胞损伤的保护作用。方法 用H2O2损伤PC12细胞建立神经元损伤模型，实验分空白细胞对照组（C组），H2O2处理组（H组），布南色林-过氧化氢处理组（B组）,阳性对照组（维生素E-过氧化氢处理组，E组）。MTT法检测细胞存活率；Hoechst33258以及TUNEL检测其对细胞凋亡影响；生化法测定超氧化物歧化酶（SOD）活性以及丙二醛（MDA）的含量。结果 与C组比较，H组的细胞存活率明显下降（P＜0.05），凋亡指数增加，细胞内的SOD活性减低，MDA水平增高（P＜0.05）；与H组比较，B组的细胞活性增强，凋亡指数下降，细胞内的SOD活性加强，MDA水平减低（P＜0.05）。结论 合适浓度的布南色林对H2O2引起的神经损伤有保护作用。

关键词: 创伤, 神经系统, 细胞凋亡, PC12 细胞, 神经保护, 布南色林, 超氧化物歧化酶, 丙二醛

Abstract: To explore neuroprotective effects of blonanserin through PC12 cells injury model induced by H2O2. METHODS Used PC12 cells treated with H2O2 as oxidative stress damage cell model. PC12 cells were divided into 4 groups: control group(C group), H2O2-treated group(H group), blonanserin treated group(B group) and positive control group(vitamin E- pretreated, E group). The viability of PC12 cells injured by H2O2 was determined by MTT assay. Used hoechst33258 stained to detect the cell apoptosis and used TUNEL assay to detect the apoptotic rate. The SOD viability and MDA levels were detecded by biochemical methods. RESULTS Comparing with the C group, the apoptotic rate and MDA levels were increased in group H (P＜0.05) , while the cell viability and the SOD viability were decreased obviously(P＜0.05). Compared with H group, the cell viability, SOD viability were significantly increased(p＜0.05), while the MDA levels and apoptotic rate were decreased(P＜0.05). CONCLUSIONS The appropriate concentration of blonanserin showed neuroprotective effect on H2O2-induced PC cells injured.

Key words: trauma, nervous system, apoptosis, PC12 cell, neuroprotective, Blonanserin, SOD, MDA