天津医药

天津医药 ›› 2016, Vol. 44 ›› Issue (9): 1099-1101.doi: 10.11958/20160013

• 临床研究 • 上一篇    下一篇

炎症因子对非 PCOS 患者颗粒细胞活性氧水平及线粒体 DNA 拷贝数的影响

张暄琳1,李毅1,刘丽1,张文静1,孟祥烔1,徐凤琴2   

  1. 天津市第一中心医院生殖医学科（邮编 300192）
  • 收稿日期:2016-01-11 修回日期:2016-04-11 出版日期:2016-09-15 发布日期:2016-09-28
  • 通讯作者: 徐凤琴 E-mail:zhangxl1218@163.com
  • 作者简介:张暄琳（1989）， 女， 硕士， 主要从事生殖医学研究
  • 基金资助:
    天津市卫生局科技基金

Effects of inflammatory markers on the level of reactive oxygen species and mitochondria DNA copy numbers in granulosa cells of patients without PCOS

ZHANG Xuanlin, LI Yi, LIU Li, ZHANG Wenjing, MENG Xiangtong, XU Fengqin   

  1. Department of Reproductive, Tianjin First Center Hospital, Tianjin 300192, China
  • Received:2016-01-11 Revised:2016-04-11 Published:2016-09-15 Online:2016-09-28
  • Contact: 徐凤琴 E-mail:zhangxl1218@163.com

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摘要： 目的 探讨炎症因子对非多囊卵巢综合征（PCOS） 患者颗粒细胞活性氧（ROS） 水平及线粒体 DNA （mtDNA）的影响。 方法 选择体外受精-胚胎移植（IVF-ET）治疗的非 PCOS 患者 50 例， 提取卵泡液中颗粒细胞进行体外培养， 随机分为炎症因子处理组和对照组， 处理组分别加入 5 nmol/L 的炎症因子白细胞介素（IL）-1、IL-6、肿瘤坏死因子（TNF）-α， 对照组加入等量炎症因子稀释液， 比较各组颗粒细胞 ROS 水平及 mtDNA 拷贝数是否存在差异。 结果 IL-1、IL-6、TNF-α 处理组颗粒细胞 ROS 水平及 mtDNA 拷贝数均显著高于对照组（ 均 P＜ 0.05）。 结论 炎症因子 IL-1、IL-6、TNF-α 可导致颗粒细胞氧化应激水平增高和线粒体受损。

关键词: 白细胞介素类, 肿瘤坏死因子 α, 活性氧, DNA, 线粒体, 颗粒细胞

Abstract: Objective To study the effect of inflammatory markers on the level of reactive oxygen species (ROS) and mitochondrial DNA (mtDNA) copy numbers in granulosa cells of patients without polycystic ovary syndrome (PCOS). Methods Fifty patients without PCOS treated with in vitro fertilization and embryo transfer (IVF-ET) were selected in this study. The granulosa cells were extracted and cultured in vitro. Cells were randomly divided into treatment group and control group. The 5 nmol/L interleukin (IL)- 1, IL- 6 and tumor necrosis factor (TNF)-α were given to treatment group, and same amount of inflammatory diluted solution was added to control group. The levels of ROS and copy numbers of mtDNA were compared between two groups. Results The ROS levels and mtDNA copy number of granulosa cells were significantly higher in IL-1, IL-6 and TNF-α treatment groups than those of control group (P＜ 0.05). Conclusion Inflammatory markers of IL-1, IL-6 and TNF-α increase the level of ROS and damage mtDNA in granulosa cells.

Key words: interleukins, tumor necrosis factor-alpha, reactive oxygen species, DNA, mitochondrial, granulosa cells