Abstract: Objective To explore the effect of cigarette smoke exposure on testis structure and ATPase enzyme activity in testis of rats. Methods Seventy adult male SD rats were randomly divided into the control group (10 rats) and the low, medium, high- dose groups (20 rats in each group, which received the exposure of 10, 20, 30 sticks of cigarettes respectively). The experimental group was exposed to cigarette smoke for 30 min per day. Each dose group was exposed for six weeks and twelve weeks (10 rats each time). The body weights in each group were recorded during the experiment. At the end of the experiment, testes of rats were removed, and the testicular tissue structure was observed by HE staining. Na +-K +- ATPase enzyme and Ca2+-ATPase enzyme activity of testicular tissue of rats were detected by enzyme-linked immunosorbent assay. Results With the increasing of exposure doses, the body weights of rats decreased (P < 0.05). The microstructure changes of testis were observed in the experimental group, and with the increasing of exposure time and exposure dose, the damage was gradually worse. After 6- week exposure, the Na +- K +- ATPase enzyme activity of testicular tissue was significantly lower in high dose group than that of rest of three groups (P < 0.05), and the Ca2 +-ATP enzyme activity was significantly lower than that of the control group (P < 0.05). The activities of Na +-K +-ATPase enzyme and Ca2 +-ATPase enzyme in testicular tissue were significantly decreased in the low, medium and high-dose groups than those of control group after being exposed for 12 weeks (P < 0.05). Conclusion Cigarette smoke exposure can retard the weight gain, cause the testis tissue damaged and decrease the activities of Na+-K +-ATPase enzyme and Ca2+-ATPase enzyme in testis of male rats.

Key words: smoking, tobacco, amog, rats, Sprague- Dawley, testis, adenosine triphosphatases, sodium- potassiumexchanging ATPase, calcium-transporting ATPases