Abstract: Objective To investigate the effect of resveratrol on autophagic flux of nasopharyngeal carcinoma CNE-2 cells, and to explore the underlying mechanism. Methods Nasopharyngeal carcinoma CNE- 2 cells were divided into control group and resveratrol group. Cells in control group were normally cultured at 37 ℃ and received no further treatment. Resveratrol group was added 40 μmol/L resveratrol 2 h before cells were culture at 37 ℃ . Western blot analysis was performed to detect protein expressions of LC3B, p62, Beclin-1, phospho-mTOR (p-mTOR) and phospho-S6 (p-S6). The autophagic flux was detected under the confocal laser scanning microscopy through different color spots, after cells were transfected with adenovirus encoding GFP- mRFP- LC3. Results (1) The protein expression of LC3B was significantly increased and the protein expression of p62 was significantly decreased in resveratrol group compared with those of control group (P＜0.05). There was no significant difference in Beclin- 1 expression between two groups. (2) Compared to control group, expressions of p- mTOR and p- S6 were significantly decreased in resveratrol group (P＜0.05). (3) Compared to control group, the red mRFP puncta were significantly increased, and the yellow GFP puncta were significantly decreased in resveratrol group (P＜0.05). Conclusion Resveratrol promotes the autophagic flux of nasopharyngeal carcinoma CNE- 2 cells, and the effects are possibly dependent on the activation of mTOR pathway-related proteins.