Abstract: Abstract: Objective To explore the treatment mechanism of severe acute pancreatitis (SAP) by adjusting the intestinal microecology of QingYiTang or Turmeric. Methods According to the random number method， forty healthy Wistar rats with SPF grade were divided into four groups, with 10 rats in each group, respectively named the control group, the model group, the QingYiTang group, and the Turmeric group. Na-Fc was injected to induce SAP model rats, before the modeling, QingYiTang and Turmeric group were respectively given corresponding traditional Chinese medicine decoctionin by tragastric administration for 1 week. The pathological changes of colon and pancreas tissues were observed by hematoxylin-eosin (HE) staining. Feces from caecal segments of rats were collected, and changes of intestinal microbial flora were analyzed by 16S rDNA sequencing technique in four groups. Results (1) According to the pathological results, the intestinal tissue of model group showed edema of intestinal wall, epithelial defect and exfoliation of intestinal mucosa, widening of interstitial space under epithelium, a large number of inflammatory cells, such as PMN infiltration. Pancreatic tissues showed pancreatic acinar edema, necrosis, enlarged septa, a large number of PMN and other inflammatory cell infiltration. The QingYiTang and Turmeric groups showed reduced pancreatic injury and intestinal mucosa damage (P＜ 0.05). (2)The results of the microecology of intestinal flora showed that the richness of firmicutes (including clostridium and bacillus), and spirulina were effectively reduced in SAP rats, otherwise the richness of bacteroides (including bacteroides and bacteroides) and lactobacillus were increased. Conclusion QingYiTang and Turmeric can increase intestinal flora diversity and richness in SAP rats, regulate the microbial ecological balance, increase the amount of probiotic group, reduce harmful bacteria colonize ability, so as to achieve the protection of intestinal function.

Key words: pancreatitis,acute necrotizing, qingyitang, turmeric, intestinal flora, 16SrDNA sequencing