Tianjin Medical Journal

Tianjin Medical Journal ›› 2020, Vol. 48 ›› Issue (5): 353-357.doi: 10.11958/20182226

• Cell and Molecular Biology •     Next Articles

Melatonin enhances the inhibitory effect of paclitaxel on T24 cell proliferation #br#

CHEN Huan1, ZHU Jia-bin2, ZHAO Xiao-jin2, DONG Chuan-jiang1,2△, DONG Zi-qiang1△ #br#   

  1. 1 Department of Urology, the First College of Clinical Medical Science of Three Gorges University, Yichang 443000, China;
    2 Department of Neurosurgery, the Affiliated Renhe Hospital of China Three Gorges University
  • Received:2019-01-03 Revised:2019-06-19 Published:2020-05-15 Online:2020-06-24

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Abstract:

Abstract: Objective To observe the inhibitory effect of melatonin (Mel) and paclitaxel (PTX) on T24 bladder cancer
cells and to explore the underlying mechanism. Methods T24 cells were treated with Mel and PTX alone or in
combination. CCK-8 assay and plate colony formation assay were used to detect the proliferation inhibition or IC50 value of
Mel and PTX on T24 cells. The mRNA and protein levels of COX-2 in T24 cells were detected by RT-PCR and Western
blot assays. The promoter activity of NF-kB was detected by dual-luciferase reporter assay. Results Compared with the
PTX treatment alone, Mel can significantly enhance the inhibitory effect of PTX on T24 cell proliferation and decrease the
IC
50 value of PTX. The proliferation and colony formation ability of T24 cells were decreased significantly (P＜0.05).
Combination therapy with Mel and PTX could significantly reduce the protein and mRNA level of COX-2 and the promoter
activity of its related NF-kB signaling pathway. Conclusion Mel and PTX could synergistically inhibit the proliferation of
T24 bladder cancer cells, which may be related to the inhibition of NF-kB signaling pathway promoter activity and the
decrease of COX-2 expression in T24 cells.

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