Effects of mild hypothermia treatment on the immune and neurological function in a swine model of extracorporeal cardiopulmonary resuscitation after cardiac arrest

doi:10.11958/20230333

Abstract

Abstract:

Objective To investigate the effect of mild hypothermia treatment (MHT) on levels of immune and neurological function indicators in a swine model of extracorporeal cardiopulmonary resuscitation after cardiac arrest (CA). Methods Eighteen Bama miniature pigs were randomly divided into the sham group, the normal temperature (NT) group and the mild hypothermia (MHT) group with 6 pigs in each group. CA model was established by right ventricular fibrillation in the NT group and the MHT group. Then cardiopulmonary resuscitationt and temperature management were followed. The target temperature of the NT group was 37 ℃, and the target temperature of the MHT group was 32-36 ℃. In the sham group, only conventional tube placement was performed, and the target temperature was 37 ℃. At 24 h after resuscitation, neurologic deficit score (NSD) was evaluated. Animals were then euthanized and venous blood sample was taken to detect brain injury and immune function indicators. Results Compared with the sham group, the NDS score increased in the NT group and the MHT group, and expression levels of neuron specific enolase (NSE) and S100B protein increased (P<0.05). Compared with the NT group, the NDS score, NSE and S100B protein expression levels were decreased in the MHT group (P<0.05). There were no significant differences in levels of IgM, IgA, IgG and T cell subsets CD4, CD8, CD4/CD8 between the groups. Conclusion Mild hypothermia treatment can significantly improve NDS, reduce brain injury after CA and has no significant effect on immune function.

Key words: hypothermia, induced, death, sudden, cardiac, cardiopulmonary resuscitation, phosphopyruvate hydratase, S100 calcium binding protein beta subunit

CLC Number:

R459.7

Figures/Tables 3

References 15

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组别	体质量/ kg	中心体温/ ℃	心率/ （次/min）	平均动脉压/ mmHg	呼吸频率/ （次/min）
Sham组	39.2±2.0	37.1±0.3	110±7	106±5	15±4
NT组	38.9±1.7	37.3±0.1	109±8	105±6	16±3
MHT组	39.3±1.9	37.2±0.2	111±7	108±4	15±4
F	0.928	0.233	0.899	0.630	0.835

组别	体质量/ kg	中心体温/ ℃	心率/ （次/min）	平均动脉压/ mmHg	呼吸频率/ （次/min）
Sham组	39.2±2.0	37.1±0.3	110±7	106±5	15±4
NT组	38.9±1.7	37.3±0.1	109±8	105±6	16±3
MHT组	39.3±1.9	37.2±0.2	111±7	108±4	15±4
F	0.928	0.233	0.899	0.630	0.835

组别	NDS/分	NSE/（μg/L）	S100B/（ng/L）
Sham组	27±9	13.4±2.2	995±56
NT组	175±34^a	42.1±3.3^a	2 102±86^a
MHT组	94±21^ab	28.5±2.9^ab	1 334±70^ab
F	59.590^＊＊	153.661^＊＊	375.803^＊＊

组别	NDS/分	NSE/（μg/L）	S100B/（ng/L）
Sham组	27±9	13.4±2.2	995±56
NT组	175±34^a	42.1±3.3^a	2 102±86^a
MHT组	94±21^ab	28.5±2.9^ab	1 334±70^ab
F	59.590^＊＊	153.661^＊＊	375.803^＊＊

组别	IgM/（g/L）		IgA/（g/L）		IgG/（g/L）
Sham组	1.64±0.22		3.49±0.30		16.12±3.22
NT组	1.71±0.16		3.57±0.21		16.35±3.06
MHT组	1.67±0.20		3.50±0.28		16.54±2.91
F	0.194		0.163		0.028
组别		CD4/%		CD8/%		CD4/CD8
Sham组		43.8±4.2		25.9±1.0		1.77±0.11
NT组		45.2±3.1		24.6±1.3		1.69±0.15
MHT组		44.5±3.5		25.5±1.1		1.72±0.13
F		0.223		2.036		0.559