Abstract: ABSTRACT：Objective To investigate the methods, safety and influence on the immune function about the treatment of dendritic cells（DC）and cytokine-induced killer cells（CIK）cultured in vitro on acute leukemia(AL). Methods Mononuclear cells (MNCs) were isolated from peripheral blood from 34 AL patients and cultured in vitro in the presence of combined cytokines to generate DC and CIK. All patients were treated with the DC and CIK by subcutaneous injection and venous transfusion. Adverse effect and safety were observed after treatment. The change of T cells subsets, the secretion of cytokines, gamma-interferon, and tumor necrosis factor, minimal residual disease were determined before and after the treatment. Results All the patients have no significant adverse effect after the treatment and continue to keep up bone marrow complete remission. The level of CD3＋CD56＋ T cells, cytokines, gamma-interferon, and tumor necrosis factor enhances after the treatment. Conclusion The review has demonstrated that this treatment is feasible and safety in AL patients and holds promise as a possible new treatment for AL patients with minimal residual disease. 【Key words】 Dendritic cells；Cytokine-induced killer；Acute leukemia；Minimal residual disease

Key words: Dendritic cells, Cytokine-induced killer, Acute leukemia, Minimal residual disease