Abstract: Abstract Objective: To investigate the effect of a disintegrin and metalloproteinase 17 (ADAM17)-siRNA on the invasion of human MCF-7 breast cancer cell by silence ADAM17 gene with siRNA. Methods: ADAM17-siRNA was transfected into MCF-7 breast cancer cells mediated by oligofectamine. ADAM17 mRNA and protein expressions were detected by real-time PCR and Western blot respectively, and the invasive ability of MCF-7 cells was studied by Transwell method. Results：ADAM17 mRNA and protein showed the high expression in MCF-7 cells, while it significantly decreased with ADAM17 siRNA treatment. Furthermore, the invasive ability of MCF-7 cells was markedly inhibited by ADAM17 siRNA. Conclusion：ADAM17 plays an important role in the invasion of MCF-7 breast cancer cells, and ADAM17-siRNA can inhibit the invasive ability of MCF-7 cell by blocking the expression of ADAM17.

Key words: Breast cancer, Adisintegrin and metalloprotease 17(ADAM17), Small interference RNA(siRNA), Invasion