Abstract: Objective：To construct eukaryotic green fluorescent protein (GFP) expressing recombinant plasmids, pEGFP-C2-hG3BP-domain (1～5), which contain domain (1～5) fragments of human G3BP. Methods：The genes of G3BP fragments were amplified by PCR from the recombinant pEGFP-C1-G3BP plasmid and inserted into pEGFP-C2 fluorescent expressing vector with EcoRI and BamHI sites. These recombinant pEGFP-C2-hG3BP-Domain(1～5) plasmids were transfected into HeLa cells and the expression of green fluorescent fusion proteins was examined by fluorescence microscope and Western blotting assay. Results：The domain (1～5) fragments of G3BP were sequenced correctly and detected in the products of the restriction single/double enzyme digestion. The green fluorescent fusion proteins were also detected in the transfected HeLa cell by fluorescence microscope and Western blotting assay. Conclusion: These recombinant eukaryotic plasmids of pEGFP- C2-G3BP (1～5) were constructed successfully and expressed effectively.

Key words: Human G3BP protein, pEGFP-C2, Recombinant plasmid, Fusion protein