Abstract: Objective： To establish and evaluate a new method to detect high producing AmpCβ-lactamase Escherichia coli.Methods:AmpC β-lactamase from 44 strains of cefoxitin-resistant Escherichia coli were detected by 3-aminophenylboronic acid (APB) disk synergy test in comparison with three-dimensional test and polymerase chain reaction (PCR). The results were analysed by statistics analysis method. Result: In 44 strains of cefoxitin-resistant Escherichia coli, AmpC β-lactamase positive rates of three methods were 70.5%，56.8% and 75% respectively.Compared with three-dimensional test, the result showed that the positive coincidence rate、negtive coincidence rate and total coincidence rate were 88.0%，52.6% and 72.7% respectively，there were no differences between APB disk synergy test and three-dimensional test（P>0.05）. Compared with PCR，the result showed that the sensitivity, specificity and the coincidence rate were 90.1%，90.1% and 90.1% respectively, there were no differences between APB disk synergy test and PCR method（P>0.05）. Conclusions:APB disk synergy test is simple,correct,not demanding special equipments. Thus ,it is applicable for routine use in clinical microbiology laboratoryes.