Bac-to-Bac baculovirus expression system in BHK cells expressing mouse IL-2 protein

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Bac-to-Bac baculovirus expression system in BHK cells expressing mouse IL-2 protein

Received:2010-09-09 Revised:2010-11-29 Published:2011-02-15 Online:2011-02-15

Abstract

Abstract: Objective The Bac-to-Bac baculovirus expression system in BHK cells expressing recombinant mouse IL-2 protein. Methods Flag to mark mouse IL-2 and join the eukaryotic cell promoter CMV, recombinant plasmid pFB-CMV-IL-2 and to the restriction enzyme digestion and PCR identification; the mouse IL-2 was cloned into the baculovirus expression vector pFastBacDual in the pFB-CMV-IL-2 was transformed into baculovirus shuttle vector containing the DH10Bac Competent Bacmid bacteria, the clones were recombinant baculovirus vector Bacmid-pFB-CMV-IL-2, extracted plasmid ; with liposome transfection method Bacmid-pFB-CMV-IL-2 transfected Sf9 insect cells packaging the virus, harvested recombinant baculovirus, will build a virus transduction of BHK cell culture, using Western Blotting Detection of IL-2 protein. Results success in BHK cells of rat recombinant IL-2 protein. Conclusion The experiment using recombinant baculovirus in mammalian cells successfully expressed recombinant mouse IL-2 protein.

Key words: mouse interleukin -2 (IL-2), baculovirus, carrier, plasmid

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Bac-to-Bac baculovirus expression system in BHK cells expressing mouse IL-2 protein

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