Abstract: Objective:To study the toxicity and radiosensitizing effect of N-(3-(3-pyridyl)acryl)-2-aminobutyric acid on hyperoxic and hypoxic Hela cells. Methods:Synthesized N-(3-(3-pyridyl)acryl)-2-aminobutyric acid with 3-pyridylacrylic acid and 2-aminobutyric acid ethyl ester hydrochloride. The survival rate and IC20 of the compound was determined by MTT assay. Sensitizing enhancement ratio(SER) was measured using colony formation test. Result:N-(3-(3-pyridyl)acryl)-2-aminobutyric acid was synthesized for the first time, the total yield was 78%. IC20 of the compound on hyperoxic and hypoxic Hela cells was 1.66 mmol/L and 1.32 mmol/L separately measured by MTT assay and SER was 1.44 and 1.84 separately by colony formation test. Conclusion:N-(3-(3-pyridyl)acryl)-2-aminobutyric acid is easy synthesized and with low cytotoxicity and radiosensitizing effect on Hela cells.

Key words: 3-pyridylacryl acid derivative, radiosensitization, colony formation test