Abstract: Abstract Objective: To elucidate new implant surface treatment methods, in order to enhance bone implant interface bonding strength and promote initial osseointegration. Methods: Pure titanium specimen submerged in HA supersaturated liquid surface processed by femtosecond laser as the test group, untreated group as control group. Surface topography of the samples was examined by SEM and the element concentration of the samples was analyzed through EDX. The samples were implanted in New Zealand rabbits femoral. After 4 weeks, making histological sections to observe experimental samples of initial bone regeneration and osteoconduction. Results: It is found that three-stage hierarchical surface structures are produced on the surface of implants which was composed of grooves, islands, nanoholes under SEM. Remarkably, Ca/P deposition were revealed on the micro-structured surfaces firmly by EDX. The surface of the control group was smooth. Histological results showed the formation of osteoid and the deposition of calcium phosphate in the text group were more active than the control group. Conclusion: The femtosecond lasers can induced topo patterns surface on pure Ti surface in HA suspension and promoted Ca/P element to deposited on the micro-patterns. Contrast with the control group, the samples processed by femtosecond laser were benefit to bone regeneration and initial stabilization.

Key words: femtosecond laser, animal experiment, histology study, osseointegration, surface topography