Abstract: Objective: The purpose of this study was to determine prolactin-inducible protein (PIP) mRNA expression, and effects of androgen in human prostate cancer cell lines. Methods: By different concentrations and different times of DHT or T, PIP and AR mRNA expression in prostate and breast tumor cell lines (LNCaP, PC-3, DU145 and T47D) were determined by RT-PCR and FQ RT-PCR. PIP protein expression in each cell lines was determined by Western blot. Results: By RT-PCR and western blot, PIP mRNA and protein expression was detected in androgen-dependent PCa cell line LNCaP. PIP mRNA expression was increase along with concentration of DHT or T in LNCaP. PIP mRNA level reach maximum peak in 10 nmol/L DHT or T. AR mRNA expression was unchanged treated by different concentration of DHT or T in LNCaP cell lines. Conclusions: The role of PIP may occur in the process of development of prostate cancer, and differentialdiagnosis prostate cancer whether or not androgen dependence.

Key words: prostate cancer, androgen, prolactin-inducible protein, real-time fluorescent quantitative RT-PCR