Abstract: Objective：To study the expression of kidney injury molecule-1（Kim-1）in rat models of acute kidney injury (AKI) induced by cis-diamminedichloroplatinum (DDP), Gentamycin(GM) or cyclosporine A. Methods：3 kinds of AKI rat models were made and renal tissue was obtaind. Using those of normal rats as control, the kidney tissue was observed for histopathology.The expression of Kim-1 mRNA was detected by RT-PCR, and that of Kim-1 protein was detected by Western Blot. Results：Compared with those in control group, histopathological observation of AKI rats indicated pathological changes at various degrees, RT-PCR proved that the level of Kim-1 mRNA in renal tissue of AKI rats was up regulated, and Western blot indicated that positive expression of Kim-1 protein in renal tissue of AKI rats was progressively increseaed. Conclusion：The increase of Kim-1 in kidney tissue directly depended on the acute kidney injury induced by DDP,GM or cyclosporine A, which supported that Kim-1 can be used as a sensitive and specific biomarker for early diagnosis of acute renal tubular injury induced by chemicals.

Key words: kidney injury molecule-1（Kim-1）, cis-diamminedichloroplatinum (DDP), Gentamycin(GM), cyclosporine A, acute kidney injury (AKI)