Abstract: Abstract: Objective: Study on acute toxicity and hypoglycemic activity of stigmata maydis extractive, and observe the hupoglycemic activity. Methods:LD50 and 95%confidence interval by Karber's method. The bloode sugar were determined by normal mice, high glucose mice that be inject glucose or adnephrin. Results: LD50 of stigmata maydis alcoh extract is 203.106(95%confidence interval is 188.665～218.652)g crude drug/kg; LD50 of stigmata maydis aqueous extract is 105.203 (95%confidence interval is 95.249～116.197)g crude drug/kg. There was no influence to normal mice. Stigmata maydis alcoh extract 30g crude drug/kg can lower blood sugar of glucose load mice ; Stigmata maydis alcoh extract and aqueous extract 30g crude drug/kg can lower blood sugar of the mice injected adr (p<0.05，p<0.01). stigmata maydis alcoh extract can add hepatic glycogen (p<0.01). Conclusion: Stigmata maydis alcoh extract and aqueous extract were atotic (LD50＞50g/kg). There was no influence to normal mice. But the two extracts can lower blood sugar of the mice injected adr, and can promote glyconeogenesis and adjust glycometabolism. To compute

Key words: Stigmata maydis extract, hypoglycemic activity, LD50