Abstract:

[Abstract]   Objective  To observe the effects of nonsteroidal anti-inflammatory drugs (NSAIDs) on the proliferation
of chondrocytes and metabolism of human osteoarthritis (OA) in short- term (48hours).   Methods  The human knee OAchondrocytes were cultured in the presence or absence of NSAIDs (celecoxib, diclofenac and ibuprofen). BrdU assays wereused to evaluate the effects of NSAIDs on the proliferation of OA chondrocytes. ELISA was used to detect the contents of proteoglycan and type-Ⅱ collagen in chondrocytes and culture supernatant. The differences of those indexes were compared between groups.   Results  The positive rate of BrdU labelling index of chondrocytes increased significantly in ibuprofen groupthan that of other groups (P<0.05). The content of type-Ⅱcollagen in the culture supernatant increased significantly in diclofenac group than that of other groups (P<0.05orP<0.01). The content of proteoglycan in the culture supernatant increased significantly in ibuprofen group than that of other groups (P＜0.05orP＜0.01). There were no significant differencesin the content of type-Ⅱcollagen and proteoglycan between chondrocytes of NSAIDs groups (P>0.05).   Conclusion   Ibuprofen may stimulate the proliferation and the secretion of proteoglycan of human OA chondrocytes. Diclofenac may stimulate the secretion of type-Ⅱcollagen of human OA chondrocytes. There were no effects of celecoxib on the proliferation andmetabolism of human OA chondrocytes.

Key words: anti-inflammatory agents, non-steroidal, osteoarthritis, chondrocytes, cell proliferation, metabolism