Abstract: Abstract Objective：With the aim to further investigate the etiology of endometrial cancer, tissue chip technology was used to evaluate the expression of Insulin-like growth factor-1(IGF-1) and cyclooxygenase-2（COX-2）in endometrial cancer tissues. Furthermore, the association between their expression and the clinicopathological features of this malignancy was also examined. Methods：The tissue chip contained 150 cases endometrial cancer examples, 54 atypical and 84 normal endometrial examples were also contained.Immunohistochemistry SP method was applied to evaluate the expression of IGF-1 and COX-2 in the tissue chip. Results：(1)The expression of IGF-1 and COX-2 in endometrial cancer were significantly higher than that in the normal examples（P<0.05）.(2)The expression of IGF-1 was positively associated with myometrial invasion（P<0.05）.No association was found between the expression of IGF-1 and histological grade, FIGO phase and lymph node metastasis. The expression of COX-2 was found positively associated with histological grade（P<0.05）.No association was found between the expression of COX-2 and myometrial invasion, FIGO phase and lymph node metastasis.(3)The effects of IGF-1 and COX-2 on the development of endometrial cancer were synergistic（P<0.05）. The expression of them was positively associated. Conclusion：With the tissue chip to evaluate the expression of IGF-1 and COX-2 in endometrial cancer ,help to earlier diagnosis of endometrial carcinoma and its pre-cancerous,comprehensive evaluation its biologic behavior, and predict invasion of biology indexes, to judge the degree malignant to have the important meaning.

Key words: endometrial carcinoma, IGF-1, COX-2, immunohistochemical method, tissuechip