Abstract:

[Abstract] Objective  To investigate the therapeutic effectiveness of transplantating hypoxic preconditioning endothelial progenitor cells (HEPCs) on acute myocardial infarction. Methods  Bone marrow endothelial progenitor cells (EPCs) were isolated from 8-week syngeneic adult male Wistar rats. After normoxic culture for 4 days, EPCs were cultured in 1% O2+ 5% CO2+94% N2 for 3 days. Then, 26 8-week syngeneic adult male Wistar rats were randomized into 3 groups: control group (n=8)，EPCs group (n=9) and HEPCs group (n=9). The acute myocardial infarction animal model was set up. Rats received 5-points peri-infarct intramyocardial injections of PBS 200 μL 2×106 EPCs and 2×106 HEPCs. After 4 weeks, the pressure-volume (P-V) curve analysis was used to evaluate the haemodynamics parameters and cardiac function. Results  After 4 weeks of transplantation, P-V loop analysis showed that there were significant differences in end diastolic volume, end systolic volume, stroke volume,ejection fraction( EF) and cardiac output between HEPCs and control groups. There were significant differences in end systolic volume, stroke volume and cardiac output between HEPCs and EPCs groups. There were no significant differences in stoke volume and cardiac output between EPCs and control groups. Conclusion Transplantation of HEPCs limited left ventricular remodeling and improved cardiac function entirely after acute myocardial infarction.

Key words: Hypoxic preconditioning, Bone marrow endothelial progenitor cells, Ischemia heart disease, Cell therapy, Pressure-volume loops