Abstract:

[Abstract] Objective  To study the influence of alcohol on osteoblast apoptosis in vitro. Methods  The mouse calvarial osteoblasts were cultured in vitro to three generations and divided into two groups: the control group and intervention group. Cells of control group were cultured in 1640 medium containing 10% FBS, and cells of intervention group were added 100 mmol/L alcohol after 1 day normal culture. After 1，3，5 and 7d culture, cells were harvested to measure MTT value. After 3，7，10 and 14 d culture, cells were harvested to measure alkaline phosphatase (ALP) and osteocalcin (BGP) activity. After 1 d culture, the osteoblast apoptosis was assessed by flow cytometry. Results  There was no significant difference in the value of MTT between two groups (P ＞ 0.05). The cellular proliferation rate was significantly lower in intervention group after 3, 5 and 7 d culture (P ＜ 0.01). The values of ALP and BGP significantly increased in control group and decreased in intervention group after 3, 7, 10 and 14 d culture (P < 0.05 orP < 0.01). The values of ALP and BGP were significantly lower in interven? tion group than those in control group after 3, 7, 10 and 14 d culture (P < 0.01). The apoptotic rate was significantly higher in intervention group than that in control group after 1 d culture (P < 0.01). Conclusion  Alcohol had a marked effect on osteo? blast apoptosis by weakening the proliferation and differentiation.

Key words: alcohol osteoblast apoptosis