Abstract: Objective To improve the detection sensitivity of serum sIgE by improve the quality of shrimp coated antigen. Methods The shrimp protein extracts were prepared and those >55KD proteins were separated by Gel Filtration Chromatography. The separated protein components were separated by SDS–PAGE and using Western blot and ELISA to evaluate whether the detection sensitivity of serum sIgE could be improved by Purified antigen.Result Those >55KD proteins ccould be successfully separated by Gel Filtration Chromatography. The SDS-PAGE of those >55KD proteins showed 10 identifiable bands. Dot-pot immunoassay showed that >55KD proteins could be improved the density spots of the weak serum. Meanwhile, the result of ELISA showed that 92.9% detection value of serum sIgE increased by coating effctive antigen. Conclusions The sensitivity of detection for serum sIgE might be improved by effective protein components of shrimp as coated antigen.

Key words: food allergy, shrimp, sIgE, Western blot, sensitivity.