Abstract: Abstract Objective: To develop an animal model of miniature pigs with coronary microembolization (CME) by easy and economical technology. Methods: A total of 11 miniature pigs were divided into control group (n=5) and CME group (n=6). Femoral artery puncture was made with a 21 gauge needle for transradial procedures. Microspheres were injected into the left anterior descending artery of the CME group by 5F coronary diagnostic catheter and 1.8F coronary micro-guide catheter. Serum concentrations of brain natriuretic peptide (BNP) and cardiac troponin I (cTnI) were evaluated just before CME and 6h after CME. Apical myocardial pathological lesions 6h after CME were evaluated by optical microscope. Results: All the miniature pigs of the control group survived, but one died in the CME group. 5F coronary diagnostic catheter and 1.8F coronary micro-guide catheter successfully arrived at the designated location. Before CME, the serum concentrations of BNP (ng/L：143.00±13.51 vs 134.00±15.57) and cTnI (μg/L：0.39±0.09 vs 0.38±0.10) showed no significant differences between these two groups (t values are 0.976 and 0.294 respectively，both P＞0.05). After CME, the serum concentrations of BNP (561.00±80.65) and cTnI (2.75±0.58) much higher than those (BNP 139.00±13.87; cTnI 0.54±0.14 ) of the control group (t values are 11.530 and 8.337 respectively，both P＜0.001). In CME group, microspheres, micro-infarction and inflammatory cell infiltration were found under an optical microscope. Conclusion: The use of new surgical consumables can successfully develop an animal model of miniature pigs with CME, and the technology is simple, economical, practical and worth promoting.

Key words: coronary microembolization, endovascular procedures, animal model, swine, miniature