Tianjin Medical Journal

Tianjin Med J ›› 2015, Vol. 43 ›› Issue (10): 1097-1099.doi: 10.11958/j.issn.0253-9896.2015.10.003

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The effect of up-regulating miR-34c-5p on the proliferation and invasion of nasopharyngeal#br# carcinoma cell line HONE-1

  

  1. 1-3, 5-6 The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China； 2 Guangdong Provincial
    Maternity and Child Care Center; 4 The First People′s Hospital of Foushan
  • Received:2015-01-23 Revised:2015-05-22 Published:2015-10-15 Online:2015-10-22

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Abstract: Abstract：Objective To investigate the effect of up-regulating miR-34c-5p on cloning formation and migration of
nasopharyngeal carcinoma cell line HONE-1 in vitro. Methods Expression levels of miR-34c-5p in nasopharyngeal cell
line NP69 and nasopharyngeal carcinoma cell line HONE-1 were investigated by real time quantitative PCR; Cloning Forma⁃
tion Test and Transwell Migration Assay were used to explore the effect of up-regulating miR-34c-5p on proliferation and
migration of nasopharyngeal carcinoma cell line HONE-1. Results The expression of miR-34c-5p in nasopharyngeal car⁃
cinoma cell line HONE-1 was lower than that in normal nasopharyngeal cell line NP69. Up-regulating miR-34c-5p signifi⁃
cantly suppressed the cloning formation and migration capacity, compared to those of NP69 cell line and HONE-1 with nor⁃
mal level of miR-34c-5p（P＜0.05） . Conclusion Down-regulating miR-34c-5p involve in proliferation and migration of
nasopharyngeal carcinoma and may be a used as a new therapeutic target for nasopharyngeal carcinoma.

Key words: nasopharyngeal neoplasms, cell proliferation, neoplasm invasiveness, in vitro , miR-34c-5p, HONE-1