Abstract:

Abstract: Objective To explore a simple and effective way of establishing a mice orthotopic tracheal transplantation
model in studying bronchiolitis obliterans to reduce operation time. Methods Tracheas from C57BL/6 mice (n=30) were im⁃
planted into Balb/c mice (n=30). Grafts and hosts were matched according to body weight. "Orotracheal intubation and endostent" was used for trachea anastomose and animal model was establish. Operating time was also noted. Grafts were harvest⁃
ed on Days 15, 30 and 60 after transplantation, using HE staining to observe pathological changes of allograft. Trachea block⁃
ing rate was also calculated. Results Until survival of trachea transplantation, the operative time of donor′s and the recipi⁃
ent′tracheas resection were (7.0±1.0) min and (7.0±1.0) min respectively. "Orotracheal intubation the endo-stent" establish⁃
ment cost（1±0.5）min. Trachea anastomose took（20.0±4.0）min and the operation time last（43.0±10.0）min. Conclusion
The improvement of orthotopic tracheas transplantation using "Orotracheal intubation the endo-sten" for trachea anastomose
is esay-operated and did required complicate skills and instruments with high successful rate. So it is an ideal and stable
model in studying bronchiolitis obliterans.

Key words: lung transplantation, bronchiolitis obliterans, disease models, animal, mice, inbred C57BL, mice, inbred
BALB/C