Abstract:

Abstract：Objective To investigate expressions of surface maturation markers, secreting cytokines and the stimulat⁃
ing effect on T lymphocytes in the dendritic cells (DC) from peripheral blood of tuberculosis (TB) patients after loading dose
treatment. Methods TB patients who received initial treatment (n=68) were collected at the fifth hospital of Shijiazhuang
from 2013 June to 2014 January. Base on clinical diagnosis and treatment guidelines of tuberculosis, they were divided into
sputum smear- negative group (35 cases) and sputum smear-positive group(33 cases). Forty cases of healthy adult were se⁃
lected as control group. Mononuclear cells were isolated from peripheral blood and were cultured in medium to differentiate
into DCs. Expression levels of CD83 and CD86 on DCs were examined by flow cytometry. The proliferation of allogeneic
mixed lymphocyte stimulated by DCs was dectected using MTT assay. Contents of IL-12, IL-10 and INF-γ in the cultural
supernatant of DCs and blood serum from TB patients were detected by ELISA. Results Compared with controls ,the ex⁃
pressions of CD83 and CD86 on DCs in TB patients after loading dose treatment decreased obviously（P＜0.05） , and the
ability to stimulate the proliferation of lymphocytes reduced evidently（P＜0.05） . What’ s more, IL-12 level increased mark⁃
edly （P＜0.05） while IL-10 and INF-γ levels presented no significant difference among the three groups (P＞0.05). Conclu⁃
sion The expressions of maturation markers of DC cells of the peripheral blood in TB patients after initial treatment de⁃
creased. The ability of stimulating mixed lymphocyte proliferation is also significantly reduced while secretion of IL-12 was
enhanced.

Key words: dendritic cells, tuberculosis, pulmonary, cytokines, flow cytometry