Abstract: Abstract: Objective　To investigate the effects of Jiawei Dachaihu decoction on liver injury and expressions of JNK and Bcl-2 in obstructive jaundice rats. Methods　Ninety SD male rats were divided into sham operation group (group S), terrier yellow group (group O) and intervention group (group M) according to random number table method, 30 rats in each group. Group S and group O were gavaged with 10 µL/g of normal saline, group M was gavaged with 10 µL/g of Jiawei Dachaihu decoction with a crude drug amount of 1 g / mL. Each group was sampled at 3 d, 7 d and 10 d after the operation, and 10 rats were randomly taken per group at each point. The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and total biliary red (TBIL) were detected in the three groups. RT-PCR and Western blot assay were used to detect mRNA and protein expressions of liver tissue JNK and Bcl-2, respectively. Results　The serum levels of AST, ALT and TBIL were significantly higher in group O and group M than those in group S, and which were significantly lower in group M than those of group O (P＜0.05). The levels of JNK mRNA and protein were significantly higher in group O and group M than those in group S, and which were significantly lower in group M than those in group O (P＜0.05). The levels of Bcl-2 mRNA and protein were significantly lower in group O and group M than those of group S (P＜0.05), and which were significantly higher in group M than those of group O (P＜0.05). Conclusion　Jiawei Dachaihu decoction can improve liver injury and promote liver tissue repairing in rats with obstructive jaundice, which may be related to the down-regulation of JNK protein, the increase of the expression level of Bcl-2 protein, the weakening of the stress of endoplasmic reticulum and the inhibition of the apoptosis of liver cells. 

Key words: Dachaihu Decoction, jaundice, obstructive, JNK mitogen-activated protein kinases, genes, bcl-2, liver injury