Tianjin Medical Journal

Tianjin Medical Journal ›› 2021, Vol. 49 ›› Issue (4): 406-409.doi: 10.11958/20202810

• Clinical Study • Previous Articles     Next Articles

Relationship between FOSL2 DNA methylation level and insulin resistance and islet β cell secretion function in type 2 diabetes mellitus of Xinjiang Uygur nationality

HOU Ze-xin1, LI Si-yuan2, CAO Guo-lei3, HU Ying4, WANG Tong-yao1, LI Jun1△   

  1. 1 Department of Endocrinology and Metabolism, the First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi 832000, China; 2 Department of Histology and Embryology, School of Medicine, Shihezi University; 3 Department of Neurology and Respiratory Medicine, Tumor Hospital Affiliated to Xinjiang Medical University; 4 Department of 
    Psychiatry, the Fourth Affiliated Hospital of Xinjiang Medical University
  • Received:2020-10-14 Revised:2020-12-09 Published:2021-04-15 Online:2021-04-16
  • Contact: LI Jun E-mail:xjlijun@163.com

PDF (PC)

4362

Abstract: Objective　To investigate the relationship between FOSL2 DNA methylation level and insulin resistance and islet β cell secretion function in type 2 diabetes mellitus of Xinjiang Uygur nationality. Methods　A total of 100 Uygur patients in the department of endocrinology and metabolism were selected as normal control group (NC group, n=50) and type 2 diabetes group (T2DM group, n=50). Biochemical indicators such as fasting plasma glucose (FPG), triglyceride (TG) and fasting serum insulin (FINS) were measured. DNA methylation level of FOSL2 was detected by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). The homeostatic model assessment index of insulin resistance (HOMA-IR) was evaluated by steady-state model. The correlation between indicators was analyzed by Spearman method, and influencing factors of insulin resistance and the secretory function of pancreatic β cells were analyzed by multiple stepwise linear regression. Results　Compared with NC group, body mass index (BMI), FPG, glycosylated hemoglobin (HbA1c), low density lipoprotein cholesterol (LDL-C), TG, total cholesterol (TC), FINS and HOMA-IR increased and high density lipoprotein cholesterol (HDL-C), homeostatic model pancreatic β cell function (HOMA-β), homeostasis model insulin sensitivity index (HOMA-ISI) decreased in T2DM group. The methylation levels of 8 cytosine-phosphor-guanine (CpG) units were increased in the T2DM group. The results of correlation analysis showed that methylation levels of 6 CpG units in FOSL2 DNA were positively correlated with HOMA-IR and negatively correlated with HOMA-ISI. The methylation levels of 4 CpG units were positively correlated with BMI and negatively correlated with HOMA-β. The results of regression analysis showed that the increased methylation level of CpG 12.13.14 was the influencing factor for the decrease of HOMA-ISI, HOMA-β and the increase of HOMA-IR. The increased methylation level of CpG 15.16.17 was the influencing factor for the increase of BMI. Conclusion　FOSL2 may be involved in insulin resistance and pancreatic β cell secretion defect in Uyghur T2DM through the elevated DNA methylation level, thus leading to the occurrence and development of T2DM.

Key words: diabetes mellitus, type 2, UYGUR NATIONALITY, DNA methylation, FOSL2, insulin resistance

CLC Number: