Expression of MMP-9 in patients with chronic rhinosinusitis and its correlation with epithelial-mesenchymal transition

doi:10.11958/20231439

Abstract

Abstract:

Objective To investigate the mechanism of matrix metalloproteinase (MMP)-9 involved in epithelial mesenchymal transformation (EMT) in chronic sinusitis (CRS). Methods The expression of MMP-9 from polypoid middle turbinate tissue was detected by immunohistochemical staining qPCR and Western blot assay in 42 patients with CRS and 8 patients underwent septoplasty. Primary human nasal epithelial cells HNEpc were cultured in vitro and divided into the control group, the TGF-β1 group (5 μg/L TGF-β1 intervention) and the TGF-β1+si-MMP-9 group (transfected with si-MMP-9 and 5 μg/L TGF-β1 intervention). The expression of MMP-9 was detected by cell immunofluorescence staining. Expression levels of TGF-β1, MMP-9 and EMT-related proteins E-cadherin, vimentin and α-SMA were detected by Western blot assay. Results (1) The positive expression rate of MMP-9 was significantly higher in the nasal mucosa of CRS with nasal polyps (CRSwNP) group (54.5%, 12/22) than that of the CRS without polyps (25.0%, 5/20) group and the control group (12.8%, 1/8). The relative expression levels of MMP-9 mRNA and protein in nasal mucosa were higher in the CRSwNP group than those in the CRSsNP group and the control group (P<0.05). (2) Compared with the control group, the expressions levels of TGF-β1, MMP-9, vimentin and α-SMA were increased in the TGF-β1 group, while the expression of E-cadherin was decreased (P<0.05). Compared with the TGF-β1 group, expression levels of TGF-β1, MMP-9, vimentin and α-SMA were decreased in the TGF-β1+si-MMP-9 group, and the expression of E-cadherin was increased (P<0.05). Conclusion The expression of MMP-9 is increased in CRS patients, which may be involved in the development of CRS through the regulation of EMT.

Key words: chronic sinusitis, MMP-9, TGF-β1, epithelial mesenchymal transformation, tissue remodeling

CLC Number:

R765.4

Figures/Tables 6

Fig.1 Expression of MMP-9 in nasal mucosa tissue of patients in 3 groups detected by immunohistochemical staining (×400)

Fig.2 Western blot analysis of MMP-9 and TGF-β1 expression in nasal mucosa/polyp tissue of patients in 3 groups

Tab.1 Changes in expression levels of MMP-9 mRNA and protein and TGF-β1 protein in the 3 groups

组别	n	MMP-9 mRNA	MMP-9蛋白	TGF-β1蛋白
对照组	8	1.00±0.03	1.00±0.02	0.21±0.03
CRSsNP组	20	1.37±0.13^a	1.14±0.06^a	0.33±0.04^a
CRSwNP组	22	1.67±0.24^ab	1.98±0.37^ab	0.68±0.15^ab
F		42.967^＊＊	76.995^＊＊	87.763^＊＊

Fig.3 Detection of MMP-9 expression by cellular immunofluorescence staining (×400)

Fig.4 Western blot analysis of expression levels of MMP-9, TGF-β1 and EMT-related proteins in the 3 groups of cells

Tab.2 Comparison of expression levels of MMP-9, TGF-β1 and EMT-related proteins between 3 groups of cells

组别	n	TGF-β1	MMP-9	E-cadherin	vimentin	α-SMA
Control组	3	0.18±0.03	0.16±0.02	0.69±0.03	0.25±0.10	0.13±0.08
TGF-β1组	3	0.74±0.16^a	0.63±0.08^a	0.29±0.06^a	0.61±0.11^a	0.64±0.16^a
TGF-β1+si-MMP-9组	3	0.13±0.04^b	0.17±0.03^b	0.48±0.04^b	0.27±0.06^b	0.27±0.09^b
F		36.737^＊＊	84.273^＊＊	64.697^＊＊	14.435^＊＊	15.584^＊＊

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