Tianjin Med J ›› 2018, Vol. 46 ›› Issue (5): 514-518.doi: 10.11958/20171103

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Study on protective effects of platelet-leukocyte aggregation on myocardial ischemia reperfusion injury and postischemic recovery in rats

SUN Jing1 , REN Fa-xin1 , SUN Xiao-jian1 , ZHANG Chuan-huan1 , LI Liu-dong1 , MU Nan2 , DONG Mei 1   

  1. 1 Department of Cardiology, 2 Department of Gynaecology and Abstetrics, Yantai Yuhuangding Hospital Affiliated to Qingdao University Medical College, Yantai 264000, China
  • Received:2017-10-17 Revised:2018-04-18 Published:2018-05-15 Online:2018-05-15

Abstract: Abstract: Objective To observe the effect of postconditioning (PostC) on the expression of platelet-leukocyte aggregation (PLA) during the process of myocardial ischemia and reperfusion in rats, and to explore the mechanisms of ischemic postconditioning (PostC) alleviating myocardial ischemia-reperfusion injury (MIRI). Methods Sixty rats were randomly divided into six groups: sham, reperfusion injury (I/R), postconditioning (PostC), SP600125 (inhibition of c-Jun N-terminal kinase, I-JNK), anisomycin and postconditioning (Ani+ PostC) and anisomycin (Ani) groups. After constructing the model of myocardial ischemia reperfusion in rats, the levels of myocardial injury markers were detected by using the CK-MB kits and TnI kits. The levels of PLA at different time points were detected by using flow cytometry. The myocardial infarction area were measured by using 2.3.5-Triphenyte-trazoliumchloride (TTC) staining, and the level of phosphorylation of JNK (P- JNK) was determined by using Western blot method. Results (1) The levels of CK-MB, TnI and the infarct size were significantly higher in the I/R group than those in the Sham group (P<0.05). The levels of CK-MB, TnI and the infarct size were significantly lower in the PostC group and I-JNK group than those in the I/R group (P<0.05). Compared with the PostC group, the levels of CK-MB, TnI and the infarct size were significantly higher in the Ani+PostC group and Ani group (P< 0.05). (2) Compared with the Sham group, the expression levels of PLA significantly increased in the I/R group at different time points after ischemia (P<0.05). At different time points of MIRI, the expressions of PLA increased gradually in I /R group, Ani+PostC group and Ani group (P<0.05). At the time point of reperfusion for 60 minutes and reperfusion for 3 hours, the expressions of PLA were significantly lower in the PostC group and I-JNK group compared with those of I/R group (P<0.05). Compared with the PostC group, the expressions of PLA were significantly higher in the Ani+PostC group and Ani group (P<0.05). (3) Compared with the Sham group, the expression levels of P-JNK were significantly higher in the I /R group (P<0.05). PostC and I-JNK inhibited the production of P-JNK (P<0.05), while Ani promoted the increase of P-JNK (P<0.05). Compared with the PostC group, the expression levels of P-JNK were significantly higher in the Ani+PostC group and Ani group (P<0.05). Conclusion PostC can reduce the expression of PLA during reperfusion by inhibiting the phosphorylation of JNK, thereby reducing myocardial ischemia-reperfusion injury.

Key words:  reperfusion injury, myocardial ischemia, disease models, animal, proto-oncogene proteins c-jun, ischemia postconditioning, platelet-leukocyte aggregation