Abstract: Objective To explore the effect of CHOP therapy on the cyclic RNA and its downstream function proteins in T cell lymphoma cells. Methods Hut78 and HH cells were treated with cyclophosphamide (1 μmol / L), hydroxydaunorubicin (0.2 μmol/L), oncovin (1 μmol/L) or prednisone (1 μmol/L) for 24 h. Conventional cultured cells were used as control. The expression levels of circRNA_001569, miR-145 and BAG4 mRNA were detected by the qPCR method, and the expression of BAG4 was detected by qPCR and Western blot assay. Results Hydroxydaunorubicin and oncovin could decrease the expression levels of circRNA_001569, promote miR-145, and down-regulate the mRNA and protein expression level of BAG4 in Hut78 and HH cell lines. Conclusion CircRNA_001569 may play an important role in the course of CHOP therapy for T cell lymphoma.

Key words: lymphoma, T-cell, antineoplastic combined chemotherapy protocols, circRNA, circRNA_001569, BAG family molecular chaperone regulator 4