Abstract: Objective　To analyze the relationship between sperm DNA fragmention index (DFI) and semen routine, sperm morphology and in vitro fertilization embryo transfer(IVF-ET) /intracytoplasmic sperm injection(ICSI) outcome, and to explore the predictive value of sperm DFI in evaluating male fertility and the outcome of IVF-ET/ICSI. Methods　Data of the sperm DFI, sperm routine and sperm morphology data of 2 420 male infertility patients, according to DFI level, the patients were divided into three groups: Group A, Group B(15%＜DFI＜30%), Group C (DFI≥30%), the correlation between sperm DFI and semen routine,malformation rate was analyzed. Sperm DFI,semen routine and sperm morphology was detected in male patients of 117 pairs of IVF-ET/ICSI infertile couples, then subdivided into IVF-1 group(DFI≤15%), IVF-2 group(15%＜DFI＜30%), IVF-3 group (DFI≥30%), ICSI-1 group(DFI≤15%), ICSI-2 group(15%＜DFI＜30%) and ICSI-3 group (DFI≥30%) according to DFI value. The effect of sperm DFI on the outcome of IVF-ET/ICSI was analyzed. Results　The age, sperm malformation rate of patients were successively increased in group A, group B and group C, while sperm concentration, PR and sperm motility rate was successively decreased (P＜0.01). The sperm DFI was positively correlated with patient age and sperm malformation rate, and the sperm DFI was negatively correlated with sperm concentration, PR and sperm motility rate (P＜0.01).Compared with IVF-1 group,the sperm concentration and PR were decreased in IVF-3 group. The sperm malformation rate was higher in IVF-3 group than that in IVF-2 group. Compared with ICSI-1 group and ICSI-2 group, the PR was decreased in ICSI-3 group. The sperm malformation rate was higher in ICSI-3 group than that of ICSI-1 group (P＜0.05). There were no statistically significant differences in fertilization rate, cleavage rate, embryo rate and clinical pregnancy rate between IVF group or ICSI group，and between all subgroups (P＞0.05). Conclusion　Sperm DFI can be used to evaluate male fertility effectively. Sperm DFI has no predictive value for IVF-ET/ICSI clinical outcomes , DFI testing of sperm is not recommended as a test for assisted reproductive technology(ART) .