Abstract: Abstract: Objective To investigate the expression of cyclophile A (Cyp A) in Gestational diabetes mellitus (GDM) and its relationship with oxidative stress, inflammation and pregnancy outcome..Methods 98 GDM patients admitted to our hospital from January 2019 to December 2020 were selected as the study group, and 40 normal pregnant women who had a pregnancy examination in our hospital during the same period were selected as the control group.According to the adverse pregnancy outcome of GDM patients, it was divided into adverse pregnancy outcome group and normal pregnancy outcome group. According to the serum CYP a level of GDM patients, it was divided into high expression group and low expression group.Serum CYP A, oxidative stress index[malondialdehyde (MDA),superoxide dismutase (SOD),8-iso-prostaglandin F2α (8-iso-PGF2α)] and inflammatory factors [interleukin-6(IL-6),High-sensitivity C-reactive protein(hs-CRP),tumor necrosis factor-ɑ (TNF-ɑ)]of all subjects were detected, and the incidence of adverse pregnancy outcomes of all GDM patients was counted.Results The levels of Fasting blood glucose (FBG), glycosylated hemoglobin (HbA1c), MDA, 8-iso-PGF2α, IL-6, hs-CRP, TNF-ɑ, and Cyp A in the study group were higher than those in the control group, and the SOD level was lower than the control group (P<0.05);The levels of FBG, HbA1c, MDA, 8-iso-PGF2α, IL-6, hs-CRP, TNF-ɑ, and Cyp A in the adverse pregnancy outcome group were higher than those in the normal pregnancy outcome group, and SOD levels were lower than the normal pregnancy outcome group (P<0.05);The incidence of macrosomia and preeclampsia in the high expression group was higher than that in the low expression group, and the difference was statistically significant (P<0.05); the incidence of neonatal hypoglycemia, premature rupture of membranes, placental abruption, and preterm delivery in the high expression group All were higher than the low expression group, but the difference was not statistically significant (P>0.05).The serum Cyp A expression level of GDM patients was positively correlated with FBG, HbA1c, MDA, 8-iso-PGF2α, IL-6, hs-CRP, and TNF-ɑ, and negatively correlated with SOD (P<0.05).Conclusion Cyp A is abnormally highly expressed in the serum of patients with GDM, and is closely related to the patient’s oxidative stress, inflammatory response and pregnancy outcome.

Key words: diabetes, gestational, cyclophilin A, oxidative stress, inflammation, pregnancy outcome