Tianjin Medical Journal ›› 2022, Vol. 50 ›› Issue (10): 1056-1060.doi: 10.11958/20220309

• Experimental Research • Previous Articles     Next Articles

The response of mouse pancreas to gradient cholecystokinin

WU Ping1(), HU Lijuan2, XU Xiaoqing1, LI Qiuju1, YAO Chuanshan3, WANG Feng2,()   

  1. 1 The Graduate School, Tianjin Medical University, Tianjin 300070, China
    2 The Laboratory of Acute Abdomen Disease Associated Organ Injury and Repair, Nankai Hospital Affiliated to Tianjin Medical University
    3 Medical School, Nankai University
  • Received:2022-03-03 Revised:2022-06-17 Published:2022-10-15 Online:2022-10-20
  • Contact: WANG Feng E-mail:pingwu@tmu.edu.cn;fengwangpi@tmu.edu.cn

Abstract:

Objective To investigate the effect of cholecystokinin (CCK) on pancreas and occurrence of pancreatic cancer, and to explore the anticancer effect of a component of green tea, epigallocatechin galate (EGCG). Methods Growing mice were randomly divided into 4 groups, and mice were given water containing soybean trypsin inhibitor (STI) 0 g/L (n=11), 2 g/L (n=11), 4 g/L (n=10) and 8 g/L (n=11), respectively. After 2 weeks, mice were sacrificed and the wet weight of pancreas was recorded. Plasma CCK level was detected by enzyme-linked immunosorbent assay (ELISA). The contents of protein, DNA, trypsin and lipase in pancreatic tissue were determined. The percentage of proliferating cell nuclear antigen (PCNA) positive cells in pancreatic tissue was detected by immunohistochemical staining. The carcinogenesis model was established by embedding dimethyl benzoanthracene (DMBA) in pancreas. Fifteen mice were randomly divided into the normal control group, the DMBA embedding group, the DMBA+2 g/L STI group, the DMBA+8 g/L STI group and the DMBA+8 g/L STI+EGCG group. After 6 weeks, the mice were sacrificed and the pancreas was taken for histological analysis. Results CCK level was higher in the 2 g/L STI group than that in the 0 g/L STI group, and which was higher in the 8 g/L STI group than that in the 0, 2 and 4 g/L STI group (P<0.05). The wet weight of pancreas was higher in the 2 g/L STI group and the 4 g/L STI group than that in the 0 g/L STI group, and which was higher in the 8 g/L STI group than that in the 0 g/L STI group and the 2 g/L STI group (P<0.05). The contents of pancreatic protein and DNA were higher in the 2 g/L STI group and 4 g/L STI group than those in the 0 g/L STI group, and which were higher in the 8 g/L STI group than those in the 0 g/L STI group, 2 g/L STI group and 4 g/L STI group (P<0.05). The content of trypsin increased successively in the 0 g/L STI group, the 2 g/L STI group, the 4 g/L STI group and the 8 g/L STI group. The lipase content was significantly higher in the 2 g/L STI group, the 4 g/L STI group and the 8 g/L STI group than that in the 0 g/L STI group (P < 0.05). The proportion of PCNA-positive cells in pancreatic tissue of mice increased successively in the 0 g/L STI group, 2 g/L STI group, the 4 g/L STI group and the 8 g/L STI group (P < 0.05). The degree of pancreatic cancer prelesion was aggravated with the increase of STI dose in drinking water, and EGCG can alleviate this lesion. Conclusion STI can induce gradient cholecystokininemia, stimulate pancreatic hyperplasia, trypsin production and pancreatic cancer, while EGCG can alleviate pancreatic precancerous lesions.

Key words: Soybean trypsin inhibitor, cholecystokinin, pancreatic growth, trypsin, lipase, Epigallocatechin gallate

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