Correlation analysis of serum Chemerin with disease activity and Th17/Treg in patients with rheumatoid arthritis

doi:10.11958/20230505

Abstract

Abstract:

Objective To explore the correlation of serum Chemerin level with disease activity and the ratio of T helper 17/regulatory T cells (Th17/Treg) in patients with rheumatoid arthritis (RA). Methods A total of 180 patients with RA who were admitted to our hospital were regarded as the observation group. According to the DAS28 score, the observation group was divided into the high activity group (60 cases), the moderate activity group (60 cases) and the low activity group (60 cases). Another 180 healthy people who underwent physical examination in our hospital during the same period were regarded as the control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum levels of Chemerin, interleukin-9 (IL-9), interleukin-10 (IL-10) and interleukin-17 (IL-17). Flow cytometry was used to detect the Th17/Treg ratio. Spearman correlation analysis was applied to analyze the correlation between serum Chemerin level and DAS28 score. Pearson correlation analysis was used to analyze the correlation between serum Chemerin level and Th17, Treg cell percentage and Th17/Treg ratio. Results The results of this study showed that the serum level of Chemerin was higher in the observation group than that in the control group (P<0.05). The serum Chemerin level was positively correlated with DAS28 score (P<0.05). Serum Chemerin levels and DAS28 scores decreased in turn in the high, moderate and low activity groups (P<0.05). The percentage of Th17 cells and the ratio of Th17/Treg were higher in the observation group than those in the control group, and the percentage of Treg cells was lower in the observation group than that in the control group (P<0.05). The level of IL-10 was lower in the observation group than that in the control group, while levels of IL-17 and IL-9 were higher in the observation group than those in the control group (P<0.05). The results of Pearson correlation analysis showed that serum Chemerin level was positively correlated with the percentage of Th17 cells and the ratio of Th17/Treg, and negatively correlated with the percentage of Treg cells (P<0.05). Conclusion Serum Chemerin level is elevated in patients with RA, which is closely related to disease activity and Th17/Treg ratio.

Key words: arthritis, rheumatoid, chemotactic factors, Th17 cells, T-lymphocytes, regulatory, interleukins, disease activity

CLC Number:

R593.22

Figures/Tables 3

References 19

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组别			CRP/（mg/L）		ESR/（mm/1 h）
对照组			1.56（0.76，2.98）		8.20（4.80，10.20）
观察组			20.50（4.10，64.00）		42.00（22.00，62.00）
Z			16.117^＊＊		9.937^＊＊
组别		类风湿因子/（IU/mL）				抗-CCP抗体/（U/mL）
对照组		18.00（13.50，24.20）				0.65（0.47，0.67）
观察组		102.00（18.00，486.00）				39.50（0.72，179.50）
Z		12.354^＊＊				23.289^＊＊
组别	补体C3/（g/L）			补体C4/（g/L）			趋化素/（μg/L）
对照组	1.05（0.92，1.21）			0.26（0.22，0.29）			50.48±15.96
观察组	1.14（0.97，1.27）			0.28（0.21，0.35）			75.69±24.25
Z或t	2.996			1.233			11.651^＊＊

组别			CRP/（mg/L）		ESR/（mm/1 h）
对照组			1.56（0.76，2.98）		8.20（4.80，10.20）
观察组			20.50（4.10，64.00）		42.00（22.00，62.00）
Z			16.117^＊＊		9.937^＊＊
组别		类风湿因子/（IU/mL）				抗-CCP抗体/（U/mL）
对照组		18.00（13.50，24.20）				0.65（0.47，0.67）
观察组		102.00（18.00，486.00）				39.50（0.72，179.50）
Z		12.354^＊＊				23.289^＊＊
组别	补体C3/（g/L）			补体C4/（g/L）			趋化素/（μg/L）
对照组	1.05（0.92，1.21）			0.26（0.22，0.29）			50.48±15.96
观察组	1.14（0.97，1.27）			0.28（0.21，0.35）			75.69±24.25
Z或t	2.996			1.233			11.651^＊＊

组别	趋化素/（μg/L）	DAS28/分
高活动组	88.40±24.56	7.89±1.72
中活动组	74.69±24.05^a	4.03±0.56^a
低活动组	63.98±24.14^ab	2.79±0.18^ab
F	15.286^＊＊	385.370^＊＊

组别	趋化素/（μg/L）	DAS28/分
高活动组	88.40±24.56	7.89±1.72
中活动组	74.69±24.05^a	4.03±0.56^a
低活动组	63.98±24.14^ab	2.79±0.18^ab
F	15.286^＊＊	385.370^＊＊

组别	Th17/%	Treg/%	Th17/Treg
对照组	0.56±0.18	4.23±1.28	0.13±0.05
观察组	1.65±0.39	3.48±0.72	0.47±0.14
t	34.046^＊＊	6.852^＊＊	30.684^＊＊
组别	IL-10/（ng/L）	IL-17/（ng/L）	IL-9/（ng/L）
对照组	27.32±3.79	5.96±0.65	6.09±0.68
观察组	17.56±3.14	13.74±2.28	13.25±2.09
t	26.605^＊＊	44.062^＊＊	43.707^＊＊