Abstract:

[Abstract]   Objective  To analyzeKlebsiella pneumoniaeby DiversiLab system, providing scientific evidence for the
control of nosocomial infection.  Methods  Eight strains of non-duplicated clinicalKlebsiella pneumoniaeisolated from the surgical ward in a hospital in2010were typed by rep-PCR-based DiversiLab system. The results were compared with those of pulsed-field gel electrophoresis (PFGE).   Results   Antimicrobial susceptibility test showed that7clinical strains were the same sensitivity to 11antimicrobial agents, except the strain K8-02. And these 7clinical strains were all multi-drug resistant. The result of PFGE showed that7strains were the same pattern. The result of DiversiLab also showed that7strains were the same pattern, and the strain K8-02was another pattern.   Conclusion   DiversiLab system is simple, quick, good repeatability and accurate, which is a kind of standardization and automation system. DiversiLab system and PFGE method could get the same result.

Key words: Klebsiellapneumoniae, electrophoresis, gel, pulsed-field, bacterial typing techniques, DiversiLab system