Abstract: Objective: To observe the protective effect of Liangge San on intestinal I/R injury. Method: A total of 168 Wistar rats were randomly divided into 5 group: normal group, sham operation group, model group, Liangge San therapeutics group and Liangge San prevention and cure group. The I/R model was made by blocking the superior mesenteric artery, and Liangge San was used to intervene. Chiu’s score, D-lactic acid content of janitrix blood plasma, MDA content and SOD activity of intestinal homogenate, apoptotic intex, the expression of the apoptotic protein Bax in the small intestine cells were measured after 45 min of ischemic and 3h、12h、24h、48h、72h after reperfusion. Results: After I/R, the intestinal morphology was significantly injuried, Chiu’s score and D-lactic acid contend of blood rised. The intestinal MDA content in model group increased and SOD activity decreased. The apoptotic index and the expression of Bax in intestinal mucosal cells of model group were obviously higher than those in other gropps. After the intervention of Liangge San, Chiu’s score and D-lactic acid content were lower than those in model group. MDA content decreased and SOD activity increased. The apoptotic index and the expression of Bax all dropped. Conclusions: Schemic reperfusion injury can destroy the function of intestinal barrier, Liangge San can protect the intestinal mucosa through cleaning the oxygen free radical.