Abstract:

[Abstract]   Objective   To investigate the effects of celecoxib on the expressions of nuclear factor-κB (NF-κB) p65
and inducible nitric oxide synthase (iNOS) in rat model of acute lung injury induced by lipopolysaccharide (LPS).  Methods   Sixty rats were randomly divided into control group, LPS group, treatment group and celecoxib group (15rats for each group).To copy the rat model of acute lung injury, LPS（5mg/kg）was injected into the tail vein in LPS group. Celecoxib (20mg/kg）was administered by gavage after30-minute modeling in treatment group. Celecoxib (20mg/kg）was also administered by gavage in celecoxib group. The same volume of normal saline was treated in control group. Rats were sacrificed after3h in four groups. The expressions of cyclooxygenase-2(COX-2), NF-κB p65and iNOS protein were detected by Western blot analysis. The expressions of NF-κB p65and iNOS mRNA were evaluated by reverse transcription polymerase chain reaction (RT-PCR) assay.  Results  Compared with control group, expression levels of COX-2, NF-κB p65, iNOS protein,NF-κB p65 and iNOS mRNA were significantly higher in LPS group (P<0.01). The NF-κB p65, iNOS mRNA and protein expressions were significantly decreased in treatment group than those of LPS group (P<0.01).   Conclusion   The selective COX-2inhibitor celecoxib has a protective effect on acute lung injury in rats.

Key words: Acutelunginjury, NF-kB, nitric oxide synthase, cyclooxygenase2inhibitors, cyclooxygenase 2