Abstract:

[Abstract] Objective  To investigate the effect of selective cyclooxygenase-2 (COX-2) inhibitor (celecoxib) on the inhibited proliferation and induced apoptosis in gastric epithelial cell transformation induced by Helicobacter pylori (H.pylori) . Methods  In vitro, the immortalized‘normal’gastric epithelial cell line GES-1 was co-cultured for two months with H. pylori Sydney strain (SS1) to establish the GES-1 transformation cell model. The cell transformation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and colony formation assay. The proliferation and apoptosis of transformed cells treated by celecoxib were detected by MTT assay and flow cytometry. Results  The proliferative activity of GES-1 cells co-cultured with H. pylori (SS1) for two months was significantly higher compared with that of control GES-1 cells. The proliferative activity of GES-1 transformed cells was inhibited with the increased concentration of celecoxib (50，75 and 100 μmol/L), and the apoptotic rate of the GES-1 transformed cells was gradually increased. Conclusion  The transformation of gastric epithelial cells can be induced by H. pylori for a long term. Celecoxib inhibits the proliferation and induce apoptosis of GES-1 transformed cells.

Key words: Helicobacter pylori, GES-1 cells, Celecoxib, Cox -2